Comparative Proteomic Analysis of <i>Shigella flexneri</i> and Its Outer Membrane Vesicle

HAN Dongmei; WEI Candong

doi:10.16211/j.issn.1004-390X(n).2017.06.002

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2017 > 32(6): 967-974. > DOI: 10.16211/j.issn.1004-390X(n).2017.06.002

HAN Dongmei, WEI Candong. Comparative Proteomic Analysis of Shigella flexneri and Its Outer Membrane Vesicle[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2017, 32(6): 967-974. DOI: 10.16211/j.issn.1004-390X(n).2017.06.002

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Comparative Proteomic Analysis of Shigella flexneri and Its Outer Membrane Vesicle

Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing 100176, China

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Received Date: July 01, 2017
Revised Date: July 12, 2017

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Abstract

Abstract

[Purpose] To gain a further understanding of the biological activity that Shigella flexneri exports via outer membrane vesicles(OMVS), we performed comparative proteomics analysis of protein profiles between bacteria and its outer membrane vesicles in response to temperature.[Method] Wild-type S. flexneir 2a strain 301 and its outer membrane vesicle respectively grown at 37 ℃ and 30 ℃were collected by sequential centrifugation. Furtherly, OMVs sample were purified by density gradient ultracentrifugation.Then the four samples were subject to shotgun proteomicanalysis by two-dimensional UPLC-linear ion trap mass spectrometry system.[Results] Total 1 510, 231,281 and 1 412 proteins were detected in bacteria itself and outer membrane vesicle grown at 37 ℃ and 30 ℃respectively.Of which, 156 proteins were common in all four samples. Further bioinformatics analysis found that there were specifically rich in COG categories of ribosomal structure and cell membrane biogenesis in the outer membrane vesicles samples. Besides,OMVs derived from strains grown at 37 ℃ specifically packaged a large number of Mxi-Spa type Ⅲ secretion system effectors.[Conclusion] Utilizing comparative proteomics technology, this study analyzed the changes of proteome of S. flexneri and its OMVs grown at different temperature for the first time and found some unique temperature-related vesicular proteins.Additionally, those selective cargo proteins detected in OMVs could serve as the groundwork for further disclosing OMV's selective package.
- Shigella flexneri,
- outer membrane vesicle,
- proteome,
- T3SS

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