Preliminary Analysis of the Effect of Exogenous Amino Acids on the Synthesis of EBP1 Protein in Arabidopsis thaliana

Purpose To investigate the types of exogenous amino acids absorbed by plants and the effect of these amino acids on the synthesis of EBP1 protein in plants.

Method We established the following system, Col-0 were grown on the 1/2 nitrogen deficient medium amended with 16 kinds of amino acids (3 mmol/L), respectively. Then, we treated the report plant 35S::EBP1-GFP with the amino acids which could be used as potential organic nitrogen sources to observe their fluorescence signals and combined with ribosome extraction essay and Western-blot essay to detect the expression of EBP1 in mRNA level and protein level, respectively.

Result There were the kinds of amino acids including arginine, alanine, asparagine, glutamine, asparagine and asparagine which could be used as potential organic nitrogen sources. It also showed that the established system was feasible. The fluorescence observation essay showed that glycine, alanine, arginine and glutarnine could promote the fluorescence signals of 35S::EBP1-GFP; the ribosome extraction essay showed that glycine, alanine and arginine significantly promoted the mRNA expression of EBP1; and the Western-blot essay showed that alanine and glutarnine promoted the accumulation of EBP1 protein at the protein level.

Conclusion Arginine, alanine, asparagine, glutamine, aspartic acid and glycine can be used as potential organic nitrogen sources for plant absorption and utilization. Moreover, alanine can promote the protein accumulation of EBP1 at the mRNA and protein levels. Exogenous amino acids regulate the growth and development of plants by affecting proteins synthesis.