Differential Proteomic Analysis of the Male Accessory Gland of Procecidochares utilis Stone under Cadmium Stress Based on iTRAQ Technique

PurposeTo clarify the effects of cadmium pollution on the proteome of the male accessory gland of Procecidochares utilis Stone.

MethodIsobaric tags for relative and absolute quantification (iTRAQ) technique and MS/MS were used to analyze and identify the proteome of the male accessory gland of P. utilis with and without cadmium.

ResultsA total of 3 661 proteins were identified, of which 58 had quantitative information differences, 38 were up-regulated and 20 were down-regulated. GO and KEGG annotation analysis and enrichment analysis were carried out on the identified differential proteins, and it was found that these proteins had catalytic, metabolic and transport functions. The results showed that the functional proteins in the male accessory glands of P. utilis were changed after cadmium-stained.

ConclusionThis study clarified the changes in the proteome of the male accessory glands of P. utilis caused by cadmium pollution, and identified the differential proteins of the male appendages of P. utilis after cadmium stress.