Determination Colonization Ability of Endophytic Actinomycetes Ac10 from Artemisia argyi in Plants

In order to study selective medium, colonization ability in plants and the effect on plant growth of endophytic actinomycetes Ac10, four antibiotics (nalidixic acid, rifampicin, penicillin, and streptomycin) and five fungicides (boscalid, pyrisoxazole, difenoconazole, sandofan, and polyoxin) were used to determine the antibiotic or fungicide resistance of Ac10. And the colonization ability and the effect on growth of hypocotyl and radicle of Ac10 in corn, tomato, cabbage, and cucumber were tested based on the selective medium. The result showed that the Ac10 could suffer the 500 mg/L polyoxin and 25 mg/L nalidixic acid or 50 mg/L penicillin. The colonization ability of Ac10 in the root, stem, and leaf of one plant were significantly different (the root the stem the leaf,P0.05),and in different plant were also significantly different (the corn the tomato the cabbage the cucumber,P0.05). There was no variation in the growth of hypocotyl or radicle of tomato, cucumber and corn treated by Ac10 suspension (P0.05).But the growth of cabbage radicle treated by Ac10 suspension was significantly increased (P0.05).Thus,there is certain feasibility about Ac10 as a biocontrol agent applying in the field.