• Chinese Science Citation Database (CSCD) Source Journals
  • A Guide to the Core Journals of China
  • Top 100 Sci-Tech Journals of Chinese Universities
  • Chinese Science and Technical Core Journals
  • China Agriculture and Forestry Core Journals (Category A)
Advanced Search
Mingying ZENG, Yongmei FU, Shu ZHANG, et al. Simultaneous Determination of 4 Flavonoids and Chlorogenic Acid of Extract from Moringa oleiferam Lam. Leaves and Its UPLC Fingerprint Analysis[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2018, 33(3): 485-493. DOI: 10.12101/j.issn.1004-390X(n).201711056
Citation: Mingying ZENG, Yongmei FU, Shu ZHANG, et al. Simultaneous Determination of 4 Flavonoids and Chlorogenic Acid of Extract from Moringa oleiferam Lam. Leaves and Its UPLC Fingerprint Analysis[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2018, 33(3): 485-493. DOI: 10.12101/j.issn.1004-390X(n).201711056
shu

Simultaneous Determination of 4 Flavonoids and Chlorogenic Acid of Extract from Moringa oleiferam Lam. Leaves and Its UPLC Fingerprint Analysis

  • 1.

    Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou 510006, China

  • 2.

    Sinopharm Group Guangdong Medi-world Pharmaceutical Co. Ltd., Foshan 528200, China

More Information
  • Received Date: November 22, 2017
  • Revised Date: January 14, 2018
  • Available Online: June 24, 2018
  • Published Date: April 30, 2018
    Graphical Abstract
    • Abstract
  • Purpose Our aim was to evaluate the similarity of the ultra performance liquid chromatography (UPLC) fingerprint of extracts from Moringa oleiferam Lam. leaves (MOLL) and to determinate the content of four flavonoid components and chlorogenic acid in extracts of MOLL from 12 different habitats.
    Methods The analysis was carried out on Phenomen Kinetex C18 (100 mm×4.6 mm, 2.6 μm) column. The mobile phase was composed of acetonitrile and 0.5% formic acid water in gradient elution mode. The flow rate was 0.35 mL/min and the detect wavelengths was 334 nm and the column was 30 ℃. The fingerprints for MOLL extracts from 12 different areas were set up. The similarity assay for MOLL extracts was carried out by Chinese Materia Medica (CMM) Fingerprint Similarity Evaluation System (2012.130723 edition), and the peaks were identified by comparing the reference substance with LC-MS/MS. External standard method was developed to determinate simultaneously contents of four flavonoid components and chlorogenic acid in the extract.
    Results UPLC fingerprints for extract of M. oleiferam leaves were established, which similarity degree was larger than 0.9, six components among twelve common peaks were identified to be chlorogenic acid (peak 3), vicenin-2 (peak 5), vitexin (peak A), isovitexin (peak 7), isoquercitrin (peak 8) and astragalin (peak 10) by UPLC-ESI-Q-TOF-MS. The average percentage composition of isoquercitrin, chlorogenic acid, astragalin, vicenin-2 and isovitexin of 12 batches of extract were respectively 6.18%, 2.28%, 2.17%, 1.38% and 0.32%. Top three habitats of the highest gross content of five components are Dongguan and Jiangmen in Guangdong, and Yuxi in Yunnan.
    Conclusion The assay method is reliable and feasible, and provides a reference method for the quantity control method of extracts from M. oleiferam leaves.
    • FullText(HTML)
    • References (12)
  • [1]
    LEONE A, SPADA A, BATTEZZATI A, et al. Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: an overview[J]. International Journal of Molecular Sciences, 2015, 16(6): 12791.
    [2]
    OLUTISHE C, KWANASHIE H, ZEZI A, et al. Chronic administration of ethanol leaf extract of Moringa oleifera Lam. (Moringaceae) may compromise glycaemic efficacy of sitagliptin with no significant effect in retinopathy in a diabetic rat model[J]. Journal of Ethnopharmacology, 2016, 194: 895. DOI: 10.1016/j.jep.2016.10.065.
    [3]
    OLAYAKI L A, IREKPITA J E, YAKUBU M T. Methanolic extract of Moringa oleifera leaves improves glucose tolerance, glycogen synthesis and lipid metabolism in alloxan-induced diabetic rats[J]. Journal of Basic and Clinical Physiology and Pharmacology, 2015, 26(6): 585. DOI: 10.1515/jbcpp-2014-0129.
    [4]
    陈瑞娇, 彭珊珊, 王玉珍, 等. 辣木叶中多糖含量的测定[J]. 时珍国医国药, 2007, 18(7): 1700. DOI: 10.3969/j.issn.1008-0805.2007.07.085.
    [5]
    吉莉莉. 辣木叶黄酮提取分离纯化及其主要成分异槲皮苷降血糖活性与机理研究[D]. 雅安: 四川农业大学, 2015.
    [6]
    WILLIAM F, LAKSHMINARAYANAN S, CHEGU H. Effect of some Indian vegetables on the glucose and insulin response in diabetic subjects[J]. International Journal of Food Sciences and Nutrition, 1993, 44(3): 191. doi: 10.3109/09637489309017439
    [7]
    KUMARI D J. Hypoglycaemic effect of Moringa oleifera and Azadirachta indica in type 2 diabetes mellitus[J]. The Bioscan, 2010, 5(20): 211.
    [8]
    GIRIDHARI V V A, MALATHI D, GEEHTA K. Anti diabetic property of drumstick (Moringa oleifera) leaf tablets[J]. International Journal of Health and Nutrition, 2011, 2(1): 1.
    [9]
    KARTHIVASHAN G, FARD M T, ARULSELVAN P, et al. Identification of bioactive candidate compounds responsible for oxidative challenge from hydro-ethanolic extract of Moringa oleifera leaves[J]. Journal of food science, 2013, 78(9): C1368. DOI: 10.1111/1750-3841.12233
    [10]
    NOUMAN W, ANWAR F, GULL T, et al. Profiling of polyphenolics, nutrients and antioxidant potential of germplasm’s leaves from seven cultivars of Moringa oleifera, Lam[J]. Industrial Crops and Products, 2016, 83: 168. DOI: 10.1016/j.indcrop.2015.12.032.
    [11]
    TAAMALLI A, ARRAEZ-ROMAN D, ABAZA L, et al. LC-MS-based metabolite profiling of methanolic extracts from the medicinal and aromatic species Mentha pulegium and Origanum majorana[J]. Phytochem Anal, 2015, 26: 320. DOI: 10.1002/pca.2566.
    [12]
    张蕾. DPP-Ⅳ抑制剂—异槲皮苷促进2型糖尿病模型小鼠胰岛素分泌及降糖作用的研究[D]. 长春: 吉林大学, 2013.
    • Related Articles

  • Related Articles

    [1]WANG Gaiping, ZHANG Lei, ZHAO Huiqin, CAO Fuliang, DING Yanpeng, WANG Zheng. Effects of Light Quality on Growth and Accumulation of Flavonoids Compounds of Ginkgo biloba Seedlings[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2024, 39(3): 163-171. DOI: 10.12101/j.issn.1004-390X(n).202307035
    [2]YANG Zhizhong, ZHANG Wenping, ZHANG Lei, GUO Dongmei, WANG Wenping. Ultrasonic-assisted Extraction of Flavonoids and Polyphenols from Clerodendranthus spicatus by Deep Eutectic Solvents and Its Antioxidant Activity[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2024, 39(3): 99-106. DOI: 10.12101/j.issn.1004-390X(n).202305002
    [3]Junyu HE, Wei ZHONG, Yunqiong CHEN, Jinglei XIONG, Weibin WANG, Yali JIANG, Huimeng SHI, Shengwei CHEN. Analysis on the Difference among the Contents of 15 Flavonoids Compound in Barley Mature Grains[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2021, 36(6): 931-936. DOI: 10.12101/j.issn.1004-390X(n).202008012
    [4]Bing JIA, Wei FAN, Geng CHEN, Ying LI, Guanghui ZHANG, Shengchao YANG, Junwen CHEN. Development of UPLC-MS Combined with HPLC Technique to Explore the Effects of Exogenous Substances on the Saponin Contents and Growth of Panax notoginseng Leaves[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(1): 116-123. DOI: 10.12101/j.issn.1004-390X(n).201712011
    [5]Le WANG, Hezhou LI, Junyu YIN, Yuan ZHANG, Zhengjie LIU, Chun LIN, Zichao MAO. The Preliminary Study on Asparagus Flavonoids Synthesis and Its Regulatory Genes[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2018, 33(2): 262-271. DOI: 10.12101/j.issn.1004-390X(n).201702045
    [6]DANG Lizhi, ZHANG Yipeng, ZHANG Jian, TAO Ying, LI Xiangzhen, DUAN Yanqing. Fast Recognized the Flavoniod Glycosides from Rosmarinus Officinalis Linn by LC-MS Technique[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2017, 32(2): 358-365. DOI: 10.16211/j.issn.1004-390X(n).2017.02.023
    [7]ZHENG Shuoli, SHAO Jianhui, ZHANG Jingli, GAO Xi, TIAN Yang, ZHANG Guanghui, YANG Shengchao, MA Chunhua. Genetic Diversity Analysis of Yunnan Moringa oleifera Lam. with AFLP Markers[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2016, 31(5): 850-855. DOI: 10.16211/j.issn.1004-390X(n).2016.05.012
    [8]XIE Wenying, CHEN Shengwei, WANG Nan, WANG Jiaxi, YANG Yingbao, WANG Le. The Difference Analysis of the Grain Flavonoid Components and Their Contents of 25 Local Barley Varieties[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2015, 30(5): 657-664. DOI: 10.16211/j.issn.1004-390X(n).2015.05.001
    [9]HU Jia kun, XIE Wenying, CHEN Shengwei, WANG Nan, YANG Yingbao, WANG Le. The Content Difference of Total Flavonoids and Anthocynin from the Grains of 25 Local Barley Varieties[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2015, 30(4): 522-527. DOI: 10.16211/j.issn.1004-390X(n).2015.04.006
    [10]WU Yijun, ZHANG Jingmei, CHEN Fang, ZHAO Yihe, YANG Yuming. Effects of Different Media on Inducing and Propagating Adventitious Buds of Moringa oleifera Lam.[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2015, 30(2): 257-262. DOI: 10.16211/j.issn.1004-390X(n).2015.02.015

  • Cited by

    Periodical cited type(6)

    1. 董文化，梅文莉，王昊，王雅丽，李薇，戴好富. 海南5个产地辣木叶的化学成分比较分析. 中国热带农业. 2022(03): 34-43+22 .
    2. 袁静，袁永兵，周铭，刘越，黄雅，王保锦，常子豪，刘宇琦，胡倩，陈尹心，周立鹏，雒晓卫，王树楷，刘乡乡，张兰珍. 基于UPLC-Q-Exactive Orbitrap-MS指纹图谱和多成分定量的不同产地辣木叶药材及其黄酮部位质量研究. 中草药. 2022(24): 7897-7904 .
    3. 段海霞，刘常青，唐晓纯，王旭龙，唐树森，彭小连. 不同产地辣木叶HPLC指纹图谱研究. 食品与药品. 2020(03): 175-179 .
    4. 赵春攀，张祖兵，段波，李海泉，龙继明. 不同种源多油辣木在西双版纳的生长表现. 热带农业科技. 2020(04): 29-32+46 .
    5. 杨芙蓉，齐耀东，刘海涛，谢彩香，宋经元. 辣木全球潜在适生区与生态特征. 北京林业大学学报. 2020(10): 45-54 .
    6. 丁丽娜，邱亦亦，束彤，阮晖. 超高效液相色谱-质谱联用技术解析沙棘果超临界CO_2萃取物中黄酮类天然产物结构. 食品科学. 2019(18): 273-280 .

    Other cited types(4)

Catalog

    Get Citation
    PDF
    XML
    Article views (1978) PDF downloads (28) Cited by(10)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles
    • Copyright © Journal of Yunnan Agricultural University Editorial Department
    • Address: Room 108, Adult Education Building, Yunnan Agricultural University, No. 95 Heijin Road, Kunming, Yunnan Postal Code: 650201
    • Tel: +86-871-65227711 E-mail: ynauzkxb@foxmail.com

    Supported by: Beijing Renhe Information Technology Co., Ltd.

    Return