Purpose Our aim was to evaluate the similarity of the ultra performance liquid chromatography (UPLC) fingerprint of extracts from Moringa oleiferam Lam. leaves (MOLL) and to determinate the content of four flavonoid components and chlorogenic acid in extracts of MOLL from 12 different habitats.
Methods The analysis was carried out on Phenomen Kinetex C18 (100 mm×4.6 mm, 2.6 μm) column. The mobile phase was composed of acetonitrile and 0.5% formic acid water in gradient elution mode. The flow rate was 0.35 mL/min and the detect wavelengths was 334 nm and the column was 30 ℃. The fingerprints for MOLL extracts from 12 different areas were set up. The similarity assay for MOLL extracts was carried out by Chinese Materia Medica (CMM) Fingerprint Similarity Evaluation System (2012.130723 edition), and the peaks were identified by comparing the reference substance with LC-MS/MS. External standard method was developed to determinate simultaneously contents of four flavonoid components and chlorogenic acid in the extract.
Results UPLC fingerprints for extract of M. oleiferam leaves were established, which similarity degree was larger than 0.9, six components among twelve common peaks were identified to be chlorogenic acid (peak 3), vicenin-2 (peak 5), vitexin (peak A), isovitexin (peak 7), isoquercitrin (peak 8) and astragalin (peak 10) by UPLC-ESI-Q-TOF-MS. The average percentage composition of isoquercitrin, chlorogenic acid, astragalin, vicenin-2 and isovitexin of 12 batches of extract were respectively 6.18%, 2.28%, 2.17%, 1.38% and 0.32%. Top three habitats of the highest gross content of five components are Dongguan and Jiangmen in Guangdong, and Yuxi in Yunnan.
Conclusion The assay method is reliable and feasible, and provides a reference method for the quantity control method of extracts from M. oleiferam leaves.
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