The Content Difference of Total Flavonoids and Anthocynin from the Grains of 25 Local Barley Varieties

The analysis result on the content difference of total flavonoids and anthocyanin from the grains of local barley varieties can be useful for selection of the barley varieties with high content of total flavonoids and anthocyanin. In the present study, a methanol solution immersion extraction was employed to extracted total flavonoids and anthocyanins from 25 local barley variety grains with different color, ultraviolet spectrophotometry was used to determinate the content of total flavonoids and anthocynin, and then the difference among total flavonoids and anthocyanin content of 25 local barley variety grains was analyzed by SPSS19.0 software. The result showed that the total flavonoids content, anthocyanin content and the percentage of anthocyanin content to total flavonoids content of 25 local barley variety grains are different significantly, their mean values were 312.37g/g, 51.05g/g, 15.32% respectively, and ranged from (151.580.69) to (629.571.26)g/g, (0.000.00) to (291.16 0.11)g/g, (0.00 0.00)% to 46.25% respectively. Anthocyanin is not the main component of total flavonoids for 25 local barley variety grains, but their content is positively correlated with purple and atropurpureus of barley grain. The barley grain color, purple and atropurpureus can be used as auxiliary selection markers in selection of barley varieties with high content of anthocynin. Among 25 local barley varieties, the barley variety, ZJ174, with high content of total flavonoids and anthocyanin, and high percentage of anthocyanin content to total flavonoids content, can be used in extraction of anthocyanin and total flavonoids, development of health food.