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XU Ling, CHEN Zihong, YANG Xiaona, et al. Effect of Different Organic Appendices on Protocorm Multiplication and Seedlings Rooting and Growth-promoting of Dendrobium officinale[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2016, 31(2): 250-256. DOI: 10.16211/j.issn.1004-390X(n).2016.02.009
Citation: XU Ling, CHEN Zihong, YANG Xiaona, et al. Effect of Different Organic Appendices on Protocorm Multiplication and Seedlings Rooting and Growth-promoting of Dendrobium officinale[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2016, 31(2): 250-256. DOI: 10.16211/j.issn.1004-390X(n).2016.02.009
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Effect of Different Organic Appendices on Protocorm Multiplication and Seedlings Rooting and Growth-promoting of Dendrobium officinale

  • Baoshan College in Yunnan, Baoshan 678000

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  • Received Date: May 20, 2015
  • Revised Date: July 10, 2015
  • Published Date: March 29, 2016
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    • Abstract
  • Organic appendices play an important role in the tissue culture of Dendrobium officinale and more organic appendices with optimum effect may be found out with broader screening. Several natural organic compounds were combined and added in the medium in the course of subculture multiplication and rooting to explore new organic appendices and combinations with promotive action. Potato juice of 100 g/L and other organic appendices of 100 g/L (banana juice, maize juice, watermelon Juice, litchi juice and wheat flour) were added in the multiplying medium. Potato juice and maize juice had optimum effect on the multiplication of protocorm (mean multiplying 5.66 times and the fresh weight enhancing 4.12 times), while the differentiation degree was low (with the 3rd differentiation rate of 67.9%), as the suitable additives in the early stage of protocorm subculture. Adding potato juice and wheat flour, the protocorm differentiation rate (mean rate 95.1%) and the increase of fresh wheat (mean 5.35 times) were the highest, and the robustness of seedlings was also the highest, as the suitable additives at the later stage of protocorm subculture. During the period of rooting and growth-promoting, banana juice of 100 g/L and other organic appendices of 100 g/L (potato juice, maize juice, watermelon juice, litchi juice and wheat flour) were added. Banana juice and wheat flour had optimum effect, mean rooting rate being 96.6%, mean root length being 4.82 cm, very significantly different with others. Both maize and wheat flour were from plant seeds and were nourishing. Their promotive effects in tissue culture of D. officinale had not been reported. The results of this study had some realistic significance to improve the seedling quality and shorten the culture cycle of D. officinale.
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    • References (18)
  • [1] 李桂锋,李进进,许继勇,等.铁皮石斛研究综述[J].中药材,2010,33(1):150.
    [2] [2] 关杰敏.铁皮石斛良种繁育与GAP种植关键技术研究[D].广州:广州中医药大学,2014.
    [3] [3] 谢寅峰,徐丽,张志敏,等.几种有机添加物对霍山石斛试管苗生理特性的影响[J].西北林学院学报,2011,26(1):77.
    [4] [4] 李春喜,王志和,王文林.生物统计学[M].2版.北京:科学出版社,2000.
    [5] [5] 何松林,孔德政,杨秋生,等.碳源和有机添加物对文心兰原球茎增殖的影响[J].河南农业大学学报,2003,37(2):151.
    [6] [6] 李小军,刘石泉,潘维陵,等.香蕉提取物对霍山石斛试管苗壮苗的影响[J].江苏大学学报(自然科学版),2004,25(6):469.
    [7] [7] 汪杰,刘小青,钱森和.不同激素水平对石斛兰组织培养的影响[J].湖南农业科学,2009(12):21.
    [8] [8] 赵兴兵,吴维佳,庞璐,等.珍稀濒危药材铁皮石斛组培快繁关键技术研究[J].湖南中医药大学学报,2012,32(3):27.
    [9] [9] 张超,张茜茜,楼楠男,等.有机添加物对大花蕙兰原球茎及幼苗生长发育的影响[J].安徽农业科学,2009,37(19):8866.
    [10] [10] 张艳,钱忠英,陈军峰,等.香蕉提取物对霍山石斛原球茎形成和小苗生长的影响[J].上海师范大学学报(自然科学版),2008,37(4):415.
    [11] [11] 李小军,刘石泉,路群,等.香蕉提取物对霍山石斛原球茎增殖的影响[J].上海师范大学学报(自然科学版),2004,33(4):74.
    [12] [12] 王蒂.植物组织培养[M].北京:中国农业出版社,2004.
    [13] [13] 徐云姬,顾道健,张博博,等.玉米果穗不同部位籽粒激素含量及其与胚乳发育和籽粒灌浆的关系[J].作物学报,2013,39(8):1452.
    [14] [14] REDENBAUGH K, SLADE D, VISS P, et al. Encapsulation of somatic embryos in synthetic seed coats[J]. HortScience, 1987, 22(5): 803.
    [15] [15] 焦顺兴,陈文龙.淀粉凝胶与植物组织培养Ⅰ.玉米淀粉凝胶培养基对苹果砧木组培分化和生长的影响[J].生态农业研究,1994,2(1):64.
    [16] [16] 焦顺兴,陈文龙,王玉珍.淀粉凝胶与植物组织培养Ⅱ.玉米淀粉凝胶培养基对苹果砧木组培生根诱导的同步化作用[J].生态农业研究,1995,3(2):18.
    [17] [17] HENDERSON W E, KINNERSLEY A M. Corn starch as an alternative gelling agent for plant tissue culture[J]. Plant Cell Tissue and Organ Culture, 1988, 15(1): 17.
    [18] [18] ZIMMERMAN R H, BHARDWAJ S V, FORDHAM I M. Use of starch-gelled medium for tissue culture of some fruit crops[J]. Plant Cell Tissue and Organ Culture, 1995, 43(3): 207.
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