PurposeTo further improve the tissue culture and rapid propagation system of Bombax ceiba, realizing the directional induction of adventitious roots of tissue culture seedlings of B. ceiba, and improving the transplanting survival rate of root seedlings of B. ceiba.
MethodsThe clustered buds of B. ceiba get from the tissue culture were used as materials. The root induction was promoted by hormone, longitudinal scratch and dark culture treatments, so as to compare the differences in the rooting rate, root number of tissue culture seedlings and transplanting survival rate under different treatments. The rooting process was observed by microscope.
ResultsThe highest rooting rate was 19.66% and 39.15% respectively when NAA or IBA was added separately, and the rooting rate was much higher when NAA and IBA used together, and the highest rooting rate was 95.80%, and the rooting seedling with one adventitious root accounted for the majority. In the treatment of longitudinal scratches, the effect of two or three scratches was better, and the rooting rate could reach more than 94%, with two adventitious roots as the majority. In dark culture, the best treatment was six days, and the rooting rate was 97.36%, and the rooting seedling with one adventitious root accounted for the majority. The microscopic observation showed that the rooting of tissue culture seedling of B. ceiba belonged to species that generate roots form cortex. In addition, there was no significant difference in transplanting survival rate among one, two or three adventitious of rooting seedling (P>0.05), but there was significant difference in biomass (P<0.05). Among them, the biomass of the transplanted seedling with three adventitious roots was the largest.
ConclusionIn this study, the rooting conditions of tissue culture seedling of B. ceiba were optimized, which provided a theoretical reference for the transplanting of tissue culture seedling of B. ceiba and the directional regulation of adventitious roots.
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