Research on Optimizing Extraction Technology of Polysaccharides from Dendrobium officinale

Purpose The purpose of this study as to optimize the extraction protocols of polysaccharides from Dendrobium officinale and the content difference of polysaccharides in D. officinale from different origins.Method The extraction efficiency was screened by orthogonal experiment with the ratio of raw materials to solvent, extracting time, and extracting frequency as factors.Results The optimal extraction technology was as followis:the ratio of raw materials to solvent was 1:40, the extracting time was 2 h, and extracting frequency was 3 times. According to the content analysis results of polysaccharides in D. officinale from Yunnan Province, where the content of the highest Menglong Jinghong reached 45.23%.Conclusion The optimum technological conditions were obtained for the extraction of polysaccharides from D. officinale, there are some differences in the content of polysaccharide in D. officinale from different habitats.