The Adventitious Bud Induction, Growth and Quality of Dendrobium officinale Kimura et Migo under Rare Earth Cerium

PurposeTo explore the methods of increasing regeneration frequency in vitro, promoting seeding growth and quality of Dendrobium officinale Kimura et Migo.

MethodMedia of tissue culture in vitro optimized were supplemented with different concentrations (0, 30, 50, 70, 90, 110, 130 and 150 μmol/L) of Ce(NO₃)₃ to explore the effects of rare earth element cerium on adventitious bud induction, growth and quality of seedling in D. officinale.

ResultAt the 70 μmol/L level of Ce³⁺, the number of buds per stem segment and the weight of buds per bottle were significantly higher than that of the control (P<0.05), both indexes were 2.77 and 4.38 times of the control respectively. There was no significant difference in SPAD value between the control and Ce³⁺ treatments (P>0.05). While the fresh weight of plants on the 70 μmol/L Ce³⁺ was significantly higher than that of the control (P<0.05), ratio of dry weight to fresh weight on 70 μmol/L Ce³⁺ treatment was 1.17 times of that of the control. The polysaccharide content of D. officinale was significantly increased by 130-150 μmol/L Ce³⁺ treatment (P<0.05), which was 24.17%-27.92% higher than that of the control. At the 70 μmol/L level of Ce³⁺, the contents of flavonoids, phenolic acid and bibenzyl reached maximum value, which increased significantly by 78.65%, 77.07% and 180.87% (P<0.05) compared with the control. According to the average value of correlation coefficients (R), the three indexes of flavonoids, phenolic acid and bibenzyl contents were correlated with other indicators, while the chlorophyll SPAD value was less correlated with other indicators.

ConclusionA certain concentration of rare earth could improve adventitious bud induction, seedling yield and quality. Therefore, appropriate concentration Ce³⁺ can be selected according to production objectives in agricultural production