Purpose To evaluate the efficiency of inclusion complex of royal jelly by β-cyclodextrin (β-CD).
Method Single factor experiment and orthogonal test were conducted, as well as investigated the qualities of the inclusion complex.
Results Temperature, pH, β-CD amount and reaction time significantly affected the soluble protein content of inclusion complex; the optimum condition of inclusion process was 27 ℃, pH 8.7, β-CD 12.50 mg/mL and 5 h. Acid, alkali and heat treatment didn’t reduce the soluble protein content of inclusion complex (P>0.05). Storing at −20 ℃ and 4 ℃ stabilized the complex quality (P>0.05). The solubility of inclusion complex was significantly higher than royal jelly (P<0.01).
Conclusion β-CD inclusion increased considerably the stability and solubility of royal jelly.