Study on the Hydrolysis of Royal Jelly Protein by Proteinase K

For studying different protease hydrolysis of royal jelly, water-soluble royal jelly was produced by proteinase K which used for biochemical test, and the optimal hydrolysis conditions of proteinase K and stability of enzymatic hydrolysates were studied in this paper. The results showed that the optimal hydrolysis of proteinase K was 55℃, pH 7.5, proteinase K 60 g/mL, CaCl2 0.01 mol/L,and time 4 h, protein extraction rate 31.03%. The stability of enzymatic hydrolysates was better. The effect of proteinase K hydrolysis of royal jelly was between plant proteinase and animal proteinase. Proteinase K could be used in the production of rich calcium type of water-soluble royal jelly.