Effects of the Stability of <i>Clostridium butyricum</i> Additive on Intestinal Microflora Regulation in Broiler Chickens

XIAO Mingxia; HUANG Zunxi; PHOUTHAPANE Vanhnaseng; ZHU Xiaodong; YUAN Lihong; CHENG Zhibin

doi:10.12101/j.issn.1004-390X(n).202206076

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2024 > 39(6): 75-81. > DOI: 10.12101/j.issn.1004-390X(n).202206076

XIAO Mingxia, HUANG Zunxi, PHOUTHAPANE Vanhnaseng, et al. Effects of the Stability of Clostridium butyricum Additive on Intestinal Microflora Regulation in Broiler Chickens[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2024, 39(6): 75-81. DOI: 10.12101/j.issn.1004-390X(n).202206076

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Effects of the Stability of Clostridium butyricum Additive on Intestinal Microflora Regulation in Broiler Chickens

1.
Yunnan Institute of Tropical Crops, Jinghong 666100, China
2.
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
3.
School of Life Science, Yunnan Normal University, Kunming 650500, China
4.
Department of Livestock and Fisheries, Ministry of Agriculture and Forestry of Laos, Vientiane 6644, Laos

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Received Date: June 28, 2022
Revised Date: October 17, 2024
Accepted Date: November 24, 2024
Available Online: November 26, 2024
Published Date: January 05, 2025

Graphical Abstract

Abstract

Abstract

Purpose
To evaluate the effects of the pelleting and storage stability of Clostridium butyricum feed additives on the growth performance and intestinal microflora in broiler chickens.
Methods
A total of 200 one-day-old Arbor Acres (AA) broiler chickens were divided into two treatment groups. The control group was fed a basic diet, while the experimental group received the basic diet supplemented with C. butyricum (1.00×10⁷ CFU/g in the complete formula feed). At 21 days of age, growth performance and the total number of Escherichia coli, Lactobacillus, and C. butyricum in cecal chyme were measured; and the antibacterial properties, the pelleting and storage stability of the C. butyricum were also evaluated.
Results
1) Compared with the control group, the average daily gain and the number of Lactobacillus of the experimental group increased significantly (P<0.05), but there was no significant difference in the feed conversion ratio and the number of E. coli (P>0.05). The number of C. butyricum in the experimental group was 2.82×10⁴ CFU/g, which was relatively low. 2) The Oxford Cup test showed a diameter of inhibition zone (DIZ) of 12.14 mm for a 1.0∶1.0 of C. butyricum to E. coli K88, but the DIZ was significantly reduced to 10.42 mm at a 0.1∶1.0 ratio, with a decrease of 14.17% (P<0.05). 3) Compared with the amount of C. butyricum added to the diet, pelleting resulted in a loss of 44.13% of C. butyricum in the experimental pellets on the 1st day, and storage for 21 days further reduced by 21.36%.
Conclusion
Dietary supplementation with C. butyricum additives can improve the growth performance and intestinal microflora of broiler chickens. However, the total number of C. butyricum significantly decrease during the pelleting and storage processes, partially affecting its ability to regulate intestinal E. coli.
- Clostridium butyricum,
- storage stability,
- pelleting stability,
- broiler chicken,
- intestinal flora

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References (36)

References

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Effects of the Stability of Clostridium butyricum Additive on Intestinal Microflora Regulation in Broiler Chickens

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