Optimization of Extraction Process for Water-soluble Protein of Moringa oleifera Leaves by Response Surface Methodology

Purpose Moringa oleifera protein content is high, but is poor water solubility, and low extraction rate. To study the extraction and increase the extraction rate of soluble protein from Moringa leaves.

Method Application of acid dissolution and ultrasonic wave assisted extraction of protein was studied by using dry Moringa leaves as a raw material by single factor experiment and response surface methodology to determine the best extraction process. The extraction of protein was investigated with response to six main variables, including ultrasonic power, ultrasonic time, extraction temperature, bath time and pH value.

Result The best extraction conditions were determined by Box-Behnken center united experimental design as follows: the ratio for material and liquid was 1∶70 (g∶mL), ultrasonic power of 300 W, Ultrasound time of 30 min, bath temperature of 53 ℃, pH value of 1.4, bath time of 60 min, the order of influence on the protein extraction rate was alkali solution pH> liquid material ratio> ultrasonic temperature> ultrasonic time,under which the yield of protein in the extracts reached (79.36±1.13) mg/g, consistent with the response surface prediction of 80.194 mg/g.

Conclusion The extraction rate of water-soluble protein was improved by this method, which was certain significance for the development of Moringa industry and the development of protein series products.