Studies on the Immobilization of Lipase with Embedding and Crosslinking Methods

XU Shan; LI Renqiang; ZHENG Zhenhua; ZHANG Yun; SUN Aijun; HU Yunfeng

doi:10.16211/j.issn.1004-390X(n).2017.06.001

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2017 > 32(6): 959-966. > DOI: 10.16211/j.issn.1004-390X(n).2017.06.001

XU Shan, LI Renqiang, ZHENG Zhenhua, et al. Studies on the Immobilization of Lipase with Embedding and Crosslinking Methods[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2017, 32(6): 959-966. DOI: 10.16211/j.issn.1004-390X(n).2017.06.001

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Studies on the Immobilization of Lipase with Embedding and Crosslinking Methods

1. Department of Biotechnology, Jinan University, Guangzhou 510632, China;
2. Department of Marine Engineering, Rizhao Polytechnic, Rizhao 276826, China;
3. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;
4. Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China

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Received Date: July 02, 2017
Revised Date: September 10, 2017

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Abstract

Abstract

[Purpose] Lipase can prepare biodiesel through catalyzing the transesterificationreactions of vegetable oils. The technology of lipase immobilization can greatly improve the efficiency of the use of lipase and reduce the cost of production.[Method] Using sodium alginate as the carrier, lipase was immobilized by the embedding-crosslinking method, and the immobilization conditions were optimized by orthogonal experiments on the base of single factor experiments.[Result] The best immobilization conditions were as follows:sodium alginate 2.5%, enzyme addition 400 U/mL, CaCl₂ 5%, and immobilization time 20 min, ethylene glycol diglycidyl ether 0.35%, crosslinking temperature 25 ℃and crosslinking time 1 h. Under above optimized conditions, the activity of immobilized lipase was about 84.90 U/g.[Conclusion] The optimal pH of immobilized lipase was 8.5, increased 0.5 U than the free enzyme, and the optimal temperature was 40 ℃, was the same with the free lipase. The thermal stability and operating stability of immobilized lipase were significantly enhanced.
- sodium alginate,
- ethylene glycol diglycidyl ether,
- lipase,
- immobilized enzyme,
- enzymatic property

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References (27)

References

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