RAPD Difference Analysis between Red and Green Stem Plants of <i>Sarcococca ruscifolia</i>

Haiyun CHEN; Zhu ZHOU; Ping BAI; Hong LI; Peiwei YONG; Tao WU; Xuexing ZHANG

doi:10.12101/j.issn.1004-390X(n).201803034

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2018 > 33(5): 898-903. > DOI: 10.12101/j.issn.1004-390X(n).201803034

Haiyun CHEN, Zhu ZHOU, Ping BAI, et al. RAPD Difference Analysis between Red and Green Stem Plants of Sarcococca ruscifolia[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2018, 33(5): 898-903. DOI: 10.12101/j.issn.1004-390X(n).201803034

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RAPD Difference Analysis between Red and Green Stem Plants of Sarcococca ruscifolia

1.
Yunnan Academy of Forestry, Kunming 650201, China
2.
Yunnan Shanchuan Landscape Limited Company, Kunming 650100, China
3.
Yunnan Province Key Laboratory of Forest Plant Cultivation and Utilization, State Forestry Administration Laboratory for Conservation of Rare, Endangered and Endemic Forest Plants, Kunming 650201, China

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Received Date: March 21, 2018
Revised Date: May 03, 2018
Available Online: September 25, 2018
Published Date: August 31, 2018

Graphical Abstract

Abstract

Abstract

Purpose The difference of total DNA between red stem plants and the green ones of Sarcococca ruscifolia was studied in order to understand the molecular basis controlling stem color.

Methods The mixed DNA template was amplified with a total of 200 RAPD primers of 10 bp, and the primers which had polymorphic bands were screened by 14 red stem plants and 14 green ones. The differential bands were retrieve, sequenced and analysed.

Results Forty-three of primers were polymorphic in 28 DNA samples, 8 of which could produce different bands in most of red and green stem plants. Three specific primers, C06, M09 and N10, could stably produce 7 distinct bands, of which band C06-350 appeared in all red stem plants, but was absent from all green ones. The sequencing result of the C06-350 showed that the sequence size was 352-381 bp and no homologous nucleotide or amino acid sequence was matched in NCBI database.

Conclusion The genetic material of the red stem type had changed indeed compared with the green one, and C06-350 was a marker closely related to the characteristics of the red stem.
- Sarcococca ruscifolia,
- red stem,
- RAPD

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References (24)

References

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