Analysis of Genetic Diversity of 20 wild Alfalfa Germplasms in Xinjiang with RAPD and ISSR Markers

Purpose In this study, RAPD and ISSR primers were used to analyze the genetic diversity of 20 wild alfalfa germplasm resources from Xinjiang.

Method RAPD and ISSR primers were screened by gradient PCR instrument and the primers with high polymorphism were selected for the study of 20 germplasm materials.

Result Six pairs of RAPD primers and ISSR primers were selected to analyze the genetic diversities of 20 alfalfa germplasms, respectively. A total of 96 bands were amplified by 6 RAPD primers, of which 91 bands were polymorphic, with a percentage of 97.85%. An average of 15.50 bands per primer was amplified. The average number of amplified polymorphic bands per primer was 15.17. A total of 157 bands were amplified by 6 ISSR primers, of which 152 bands were polymorphic, with a percentage of 96.82%. An average of 26.17 bands per primer was amplified, and each primer amplified an average of 25.33 polymorphic bands. The range of Nei’s gene diversity was 0.176 3−0.274 0 with an average of 0.207 1 and 0.085 0−0.154 1 with an average of 0.113 3 for RAPD and ISSR markers, respectively.

Conclusion The results of cluster analysis showed that there was a certain correlation between germplasm clustering and geographical origin.