Identification and Characteristic Analysis of Wheat CesA Gene Family

PurposeTo systematically identified and analyzed wheat cellulose synthase (CesA) gene family members, laying a theoretical foundation for further elucidating their biological functions.

MethodsThe wheat CesA (TaCesA) gene family members were comprehensively identified on the whole wheat genome using bioinformatics methods. The phylogeny, chromosome position, and transcriptome of TaCesA genes were analyzed. Then, TaCesAs gene structure, conserved motifs, cis-elements, protein characteristics, and subcellular location were predicted. Moreover, CesA homologous genes between wheat and its subgenomic donors were compared.

ResultsA total of 21 TaCesA genes were identified, divided into three groups (a, b and c). The TaCesAs contained multiple introns, but the untranslated region (UTR) structure of some genes was missing. The relationship between the TaCesA genes and their subgenome donors was conserved. The upstream of TaCesAs cover 45 cis-acting elements related to biological/abiotic stress, growth and development, and plant hormones, suggesting that these genes might be involved in the biological function respondings of wheat. The expression profile results showed the TaCesA genes were responsive to phosphorus deficiency, high temperature, low temperature, drought, stripe rust, powdery mildew, Fusarium graminearum, and other stresses.

ConclusionThe wheat CesA genes have conserved gene structure and protein structure, and they play an essential role in wheat resistance to abiotic and biological stresses.