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Ping TANG, Pengfei HE, Yuan YUAN, et al. Colonization of the Biocontrol Bacterial Strain DJB5 against Aspergillus flavus in Maize Grains[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2020, 35(3): 409-414. DOI: 10.12101/j.issn.1004-390X(n).201905036
Citation: Ping TANG, Pengfei HE, Yuan YUAN, et al. Colonization of the Biocontrol Bacterial Strain DJB5 against Aspergillus flavus in Maize Grains[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2020, 35(3): 409-414. DOI: 10.12101/j.issn.1004-390X(n).201905036
shu

Colonization of the Biocontrol Bacterial Strain DJB5 against Aspergillus flavus in Maize Grains

  • 1.

    College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China

  • 2.

    College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China

  • 3.

    National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650217, China

More Information
  • Received Date: May 14, 2019
  • Revised Date: March 20, 2020
  • Available Online: June 07, 2020
  • Published Date: April 30, 2020
    Graphical Abstract
    • Abstract
  • PurposeTo understand the colonization of the biocontrol bacterial strain DJB5 in maize grains and its regularities.
    MethodPlasmid pHT01-P43GFPmut3a was transformed into DJB5 cells for fluorescence tagging by natural transformation. The stability of plasmid, growth tendency and biofilm-forming ability of DJB5-GFP were tested, and the control efficiency difference between DJB5 and DJB5-GFP strains was analyzed using plate antagonism bio-assay and grain storage experiment.
    ResultPlasmid pHT01-P43GFPmut3a could keep stable, and its introduction had no significant negative load on DJB5 growth and biocontrol activity. The storage experiment showed that under different grain moisture contents and storage temperatures, the number of DJB5-GFP increased with the increase of grain moisture contents and was as high as 107 CFU/g (orders of magnitude) at the grain moisture content of 30%; colonization was greater at storage temperature ranging from 28 ℃ to 35 ℃ than that below 28 ℃ and above 35 ℃. The result indicated that DJB5 had the potential for controlling Aspergillus flavus on maize grains during post-harvest storage.
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    • References (23)
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