FRBI Down-regulates <i>K-Ras</i> and <i>c-Myc</i> Genes of Ovarian Cancers and IP3 Signal Pathway

Chen WANG; Juan YANG; Luju LAI; Zhaofang YUAN; Suocheng WEI

doi:10.12101/j.issn.1004-390X(n).201809001

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2019 > 34(1): 22-28. > DOI: 10.12101/j.issn.1004-390X(n).201809001

Chen WANG, Juan YANG, Luju LAI, et al. FRBI Down-regulates K-Ras and c-Myc Genes of Ovarian Cancers and IP3 Signal Pathway[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(1): 22-28. DOI: 10.12101/j.issn.1004-390X(n).201809001

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FRBI Down-regulates K-Ras and c-Myc Genes of Ovarian Cancers and IP3 Signal Pathway

College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China

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Received Date: September 01, 2018
Revised Date: December 11, 2018
Available Online: January 07, 2019
Published Date: December 31, 2018

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Abstract

Abstract

Purpose To investigate the effects of follicle stimulating hormone receptor binding inhibitor (FRBI) on K-Ras, c-Myc and FSHR mRNA and protein levels in sheep oocytes, and further clarify whether FRBI works through the IP3 signaling pathway.

Methods Oocytes were cultured for 24 h in an in vitro maturation medium at 10 IU/mL of FSH and different mass concentrations of FRBI (0, 10, 20, 30 and 40 μg/mL). The contents of K-Ras, c-Myc, cAMP, FSH and IP3 in IVM culture medium were determined by ELISA. The qRT-PCR and Western-blot were used to detect the levels of FSHR mRNA and protein in oocytes.

Results At 24 h, along with the increase of FRBI dose, the mass concentration of c-Myc in COM-1 group to COM-3 group gradually decreased, COM-3 was significantly lower than that in FSH group (P<0.05), and the K-Ras mass concentration in COM-4 group was 24 h and significantly lower than the negative control group (CG) and FSH group (P<0.05); in the COM group,FSHR mRNA and protein expression levels decreased with increasing FRBI dose, high dose group (COM-4) mRNA and protein expression levels significantly lower than the FSH group (P<0.05). The contents of cAMP and IP3 decreased with increasing dose of FRBI, and IP3 in COM-4 group was significantly lower than CG group (P<0.05).

Conclusions In the presence of FSH, FRBI could reduce the production of K-Ras and c-Myc, inhibit the FSHR level of oocytes. FRBI exerts its functions probably through the IP3 signaling pathway.
- FSH receptor binding inhibitor,
- FRBI,
- ovarian cancer,
- K-Ras,
- c-Myc,
- IP3

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References (24)

References

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