Polymorphisms of the Exon 5 in <i>PRKAG3</i> Gene and Its Association with Meat Quality Traits of Xuanhe Pigs

Wei HU; Xinxing DONG; Kezhong ZHANG; Jiashu HE; Dawei YAN; Hailian LI; Mingli LI; Shaoxiong LU

doi:10.12101/j.issn.1004-390X(n).201909036

JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science） > 2020 > 35(1): 26-32. > DOI: 10.12101/j.issn.1004-390X(n).201909036

Wei HU, Xinxing DONG, Kezhong ZHANG, et al. Polymorphisms of the Exon 5 in PRKAG3 Gene and Its Association with Meat Quality Traits of Xuanhe Pigs[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2020, 35(1): 26-32. DOI: 10.12101/j.issn.1004-390X(n).201909036

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Polymorphisms of the Exon 5 in PRKAG3 Gene and Its Association with Meat Quality Traits of Xuanhe Pigs

1.
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
2.
Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Kunming 650201, China
3.
Xuanwei Livestock and Poultry Improvement Station, Xuanwei 655400, China

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Received Date: September 26, 2019
Revised Date: November 14, 2019
Available Online: December 23, 2019
Published Date: December 31, 2019

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Abstract

Abstract

PurposeThe aim of the present study was to investigate the polymorphisms of the exon 5 in protein kinase adenosine monophosphate gamma 3 subunit (PRKAG3) gene and its association with meat quality traits in Xuanhe pigs.
MethodThe single nucleotide polymorphisms (SNPs) within the exon 5 of PRKAG3 gene were detected by PCR product directed sequencing in 120 Xuanhe pigs, the effects of single- locus and multiple-loci combined genotypes of the identified SNPs on 6 meat quality traits were analyzed by using least square models.
ResultThree SNPs were identified within the exon 5 of PRKAG3 gene, which included 2 synonymous mutations (T579C, T580C) and 1 missense mutation (G595A), which were all in Hardy-Weinberg equilibrium. For the loci T579C, T580C and G595A, the highest frequency was presented for the genotypes TT, TT, GA and the alleles T, T, G, respectively. The genotypes TT and TC of T579C and T580C could significantly increase the marbling score (MS) (P<0.05) and decrease the water loss rate (WLR) (P<0.01), which presented as significant additive and additive-dominance effects (P<0.05 or P<0.01); pigs with genotype AA on locus G595A had the lowest WLR and the highest cooked rate (P<0.05), which presented as significant additive effects (P<0.05); the three-loci combined genotypes TTTTGG, TCTCGG and TTTTAA were the highest on MS and pH₁, and lowest on WLR (P<0.05 or P<0.01), respectively; but the genotype CCCCGG was the lowest on MS and pH₁, and highest on WLR (P<0.05 or P<0.01).
ConclusionThe present results further confirmed the polymorphisms in the exon 5 of PRKAG3 gene were significantly associated with the pork MS, pH and WLR in Xuanhe pigs, which the synergistic effects were presented for the genotype CC of T579C/T580C and the genotype GG of G595A on decreasing the meat quality.

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[1]	鲁绍雄, 连林生, 李庆科, 等. 基于乌金猪深度发掘的优质高效新品种选育[J]. 猪业科学, 2016, 33(12): 120. DOI: 10.3969/j.issn.1673-5358.2016.12.038.
[2]	HARDIE D G, SCOTT J W, PAN D A, et al. Management of cellular energy by the AMP-activated protein kinase system[J]. FEBS Letters, 2003, 546(1): 113. DOI: 10.1016/S0014-5793(03)00560-X.
[3]	CARLING D. The AMP-activated protein kinase cascade: a unifying system for energy control[J]. Trends in Biochemical Sciences, 2004, 29(1): 18. DOI: 10.1016/j.tibs.2003.11.005.
[4]	KAHN B B, ALQUIER T, CARLING D, et al. AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism[J]. Cell Metabolism, 2005, 1(1): 15. DOI: 10.1016/j.cmet.2004.12.003.
[5]	HARDIE D G. AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy[J]. Nature Reviews Molecular Cell Biology, 2007, 8(10): 774. DOI: 10.1038/nrm2249.
[6]	BARNES B R, MARKLUND S, STEILER T L, et al. The 5'-AMP-activated protein kinase γ3 isoform has a key role in carbohydrate and lipid metabolism in glycolytic skeletal muscle[J]. Journal of Biological Chemistry, 2004, 279(37): 38441. DOI: 10.1074/jbc.M405533200.
[7]	MILAN D, JEON J T, LOOFT C, et al. A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle[J]. Science, 2000, 288(5469): 1248. DOI: 10.1126/science.288.5469.1248.
[8]	CIOBANU D, BASTIAANSEN J, MALEK M, et al. Evidence for new alleles in the protein kinase adenosine monophosphate- activated gamma (3)-subunit gene associated with low glycogen content in pig skeletal muscle and improved meat quality[J]. Genetics, 2001, 159(3): 1151. DOI: 10.1023/A:1013367100865.
[9]	OTTO G, ROEHE R, LOOFT H, et al. Associations of DNA markers with meat quality traits in pigs with emphasis on drip loss[J]. Meat Science, 2007, 75(2): 185. DOI: 10.1016/j.meatsci.2006.03.022.
[10]	RYAN M T, HAMILL R M, O’HALLORAN A M, et al. SNP variation in the promoter of the PRKAG3 gene and association with meat quality traits in pig[J]. BMC Genetics, 2012, 13(1): 66. DOI: 10.1186/1471-2156-13-66.
[11]	DALL' OLIO S, SCOTTI E, COSTA L N, et al. Effects of single nucleotide polymorphisms and haplotypes of the protein kinase AMP-activated non-catalytic subunit gamma 3 (PRKAG3) gene on production, meat quality and carcass traits in Italian Large White pigs[J]. Meat Science, 2018, 136: 44. DOI: 10.1016/j.meatsci.2017.09.012.
[12]	LINDAHL G, ENFÄLT A C, VON SETH G, et al. A second mutant allele (V199I) at the PRKAG3 (RN) locus—I. Effect on technological meat quality of pork loin[J]. Meat Science, 2004, 66(3): 609. DOI: 10.1016/S0309-1740(03)00179-7.
[13]	LINDAHL G, ENFÄLT A C, VON SETH G, et al. A second mutant allele (V199I) at the PRKAG3 (RN) locus—Ⅱ. Effect on colour characteristics of pork loin[J]. Meat Science, 2004, 66(3): 621. DOI: 10.1016/S0309-1740(03)00180-3.
[14]	UIMARI P, SIRONEN A. A combination of two variants in PRKAG3 is needed for a positive effect on meat quality in pigs[J]. BMC Genetics, 2014, 15(1): 29. DOI: 10.1186/1471-2156-15-29.
[15]	鲁绍雄, 连林生. SAS统计分析系统在畜牧科学中的应用[M]. 昆明: 云南科技出版社, 2003.
[16]	HUANG L S, MA J W, REN J, et al. Genetic variations of the porcine PRKAG3 gene in Chinese indigenous pig breeds[J]. Genetics Selection Evolution, 2004, 36(4): 481. DOI: 10.1186/1297-9686-36-4-481.
[17]	CHEN J F, DAI L H, PENG J, et al. New evidence of alleles (V199I and G52S) at the PRKAG3 (RN) locus affecting pork meat quality[J]. Asian-Australasian Journal of Animal Sciences, 2008, 21(4): 471. DOI: 10.5713/ajas.2008.70373.
[18]	UIMARI P, SIRONEN A, SEVÓN-AIMONEN M L. Evidence for three highly significant QTL for meat quality traits in the Finnish Yorkshire pig breed[J]. Journal of Animal Science, 2013, 91(5): 2001. DOI: 10.2527/jas.2012-5811.
[19]	ZHANG C Y, WANG Z Q, BRUCE H, et al. Genome-wide association studies (GWAS) identify a QTL close to PRKAG3 affecting meat pH and colour in crossbred commercial pigs[J]. BMC Genetics, 2015, 16(1): 33. DOI: 10.1186/s12863-015-0192-1.
[20]	VERARDO L L, SEVÓN-AIMONEN M L, SERENIUS T, et al. Whole-genome association analysis of pork meat pH revealed three significant regions and several potential genes in Finnish Yorkshire pigs[J]. BMC Genetics, 2017, 18(1): 13. DOI: 10.1186/s12863-017-0482-x.
[21]	ENFÄLT A C, VON SETH G, JOSELL Å, et al. Effects of a second mutant allele (V199I) at the PRKAG3 (RN) locus on carcass composition in pigs[J]. Livestock Science, 2006, 99(2/3): 131. DOI: 10.1016/j.livprodsci.2005.06.007.

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Polymorphisms of the Exon 5 in PRKAG3 Gene and Its Association with Meat Quality Traits of Xuanhe Pigs

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