Abstract: The chemistry is an experimental science. College enrollment, the price rise of chemical reagents and the inadequacy of laboratories made the traditional teaching of chemical experiment difficult to adapt the education system innovation and the development of modern university, so the teaching reform of chemistry experiment was inevitable. With the reality of South China Agricultural University, one of the directions in teaching reform of chemical experiment is to investigate and apply microscale chemical experiment. The historical development, the problems and the development direction of microscale chemical experiment are discussed, which has reference value to the reform of experiment of other subjects.