Abstract: Botany is one of the important basic courses which belongs to the majors of plant production in agricultural universities.Botanical experiment is the critical link which is indispensable for the whole teaching procedure of botany. However, being provided with many disadvantages, the traditional teaching modes of botanical experiment are difficult to satisfy the needs of the development of botany and the quality education in universities. The main aspects of the teaching reform of botanical experiment should include: setting up botanical experiment course independently, strengthening the hardware construction of botanical laboratory, renovating the teaching material and integrating the contents of botanical experiment course, educing the demonstration effects of teachers really, applying multimedia technology and micro interaction system in teaching activities energetically to endeavor to realize the interactions between the teacher and the students, and establishing the consummate examining system of botanical experiment course.