Abstract: For cultivating applied undergraduate talents, the project-based teaching method has been used in undergraduate thesis course for the tobacco-major students, through the procedure of how to design the project tasks, prepare projection, implement project, how to realize inspection and evaluation by students themselves, how to improve self-learning ability, observation ability, practical ability, scientific research ability, and the comprehensive ability such as team cooperation, communication and exchanges, in order to significantly improve the teaching of undergraduate thesis course. In this paper, we applied case analysis, dissecting deeply all aspects of project teaching method, discussing and evaluating the effect, in order to relentlessly improve teaching methods and make beneficial exploration for cultivating applied talents in tobacco science.