A reasonable role of grassroots government can improve its comprehensive governance ability and thus ensure the smooth implementation of rural revitalization strategy. However, in the new stage, there are role conflicts and contradictions in different dimensions of grassroots government. This study coded and analyzed the textual data related to the role of grassroots government under the threshold of rural revitalization through the rooting theory, and formed a three-dimensional analysis framework of situation-conflict-adjustment of the role of grassroots government under the threshold of rural revitalization based on the construction of a role conflict model. The study found that the “role conflict” of grassroots government in the context of rural revitalization has both individual characteristics and specific situations. The role conflict of grassroots government is mainly reflected in role cognition-competence conflict, role structure-institutional conflict and role field-subject conflict. Under the dual attributes, the role conflict of grassroots government should be adjusted from three dimensions: policy shaping behavior mechanism, organization cultivating ability mechanism and system reshaping structure mechanism. By analyzing and adapting the role conflict behaviors, it provides some reference values for the functional orientation of grassroots government and the steady promotion of rural revitalization strategy.