Abstract: This project takes fresh-cut flowers as the main research object, through literature collection and the field investigation, it summarizes and analyzes the overall import and export situation of fresh cut flowers in Yunnan in recent years, especially the import and export trade volume of fresh-cut flowers between Yunnan and Southeast Asia and South Asia, as well as the trade development status and change trend of trading countries. The paper predicts the development prospect and trend of fresh-cut flower trade between Yunnan and southeast Asia and South Asia in the future according to the policy background of “One Belt and One Road”. On this basis, it focuses on the current problems existing in the cut-flowers in Yunnan's foreign trade, and puts forward suggestion which is about the cut-flower production supply chain, sales and transportation related measures. It is expected to further enhance the export ability of Yunnan fresh cut flowers to countries along the “One Belt and One Road”, improve the competitiveness of Yunnan flowers in the international market, promote the fresh cut flowers trade between Yunnan and Southeast Asia and South Asia countries, and realize mutual benefit and win-win situation.