Abstract: The construction of new agricultural science urgently requires us to speed up the innovation of practical teaching to promote the comprehensive development of students. By analyzing the advantages and disadvantages of existing mixed teaching and the advantages of situational mixed teaching based on PBL, this paper explores the construction of situational mixed teaching mode based on PBL and estimates its application effect in the case of animal physiology experiment. The results showed that students ’ test scores in the situational mixed teaching group based on PBL were significantly higher than those in the general mixed teaching group. According to the quantitative questionnaire, PBL-based mixed teaching group students believe that the situational mixed teaching group based on PBL greatly improved their learning interest, innovation consciousness, teamwork, problem analysis and solving, communication and other abilities. It shows that the teaching model has good effect and is worthy of further study and promotion.