Abstract: The rational operation of rural tourism professional cooperatives as a new model of rural tourism will be conducive to promote the sustainable development of rural tourism. This paper chooses the“Wujiafeng Rural Tourism Professional Cooperative”in Huixian Village of Yunnan Province as the case study, uses the actor-network-theory to clarify the participants and their compulsory access points, combs the tasks and objectives of various stakeholders, and constructs a dynamic actor network alliance through the process of translation. Finally, the improvement measures are put forward, so as to provide more novel theoretical research paradigms for rural tourism development.