Abstract: In order to objectively evaluate the demand of science and technology service of the new agricultural business subjects, the article analyzes the demand characteristics by methods of material analysis, field research, combination of statistic analysis and theoretical research. The result shows that there are 76.11% of the new agricultural business subjects in urgent need, while 7.08% of whom are indifferent with scientific and technological services; their demand for technical guidance is far greater than other indicators, demand rate of agricultural enterprises, professional cooperative organizations and family farms for technical guidance reached 52.08%, 82.14%, 88.89%, respectively;91.15% of the new agricultural business subjects prefers public welfare services. Therefore, this article puts forward countermeasures and suggestions from scientific research project of top-level design, the construction of agricultural extension system, cultivation of demand type research results, strengthen service brand awareness so as to provide theoretical reference for other units to carry out related work.