Abstract: International cooperation is a developing mode of cooperation, and University is the main body in the process. International cooperation in education and science and technology is an important part of University internationalization, so Yunnan college and universities should give full play of its geographical advantages and technological advantages, and cooperate with enterprises in Southeast Asia countries in education and science and technology, and drive the technology industry to promote the development of regional economy. Based on the analysis of existing literature results and questionnaire investigation, in order to realize the cooperation between the two sides smoothly, We must construct the practical security system, including policy guarantee, Organizational guarantee, platform construction security, management system and guarantee, its capacity building and manpower support. The various security conditions are not isolated, on the contrary, they are interdependence, mutual restriction and mutual influence. The effective operation of the security system can promote the development of cooperation in education and science and technology smoothly.