Abstract: Supply chain finance provides new ideas for the problem of financing difficulties for agriculture-related enterprises. In this regard, the article selects agricultural enterprises listed in China’ s A-shares from 2014 to 2024 as research samples, and adopts the fixed effect model to conduct an empirical analysis on the correlation between supply chain finance and financing constraints. It also examines the mediating role of credit risk and information asymmetry in their relationship and further analyzes the impact of differences in supply chain concentration and industry background. The research finds that supply chain finance has a significant improvement effect on the financing difficulties of agriculture-related enterprises and can effectively alleviate their financing pressure. Moreover, by reducing the level of credit risk and the degree of information asymmetry, supply chain finance can further enhance its effectiveness in alleviating the financing predicament of agricultural enterprises. A deeper analysis reveals that in agricultural enterprises with stable supply chain structures and within the manufacturing industry, supply chain finance shows more significant effects in easing financing constraints. This article expands the research dimension of financing constraints for agricultural enterprises and provides new theoretical basis for different types of enterprises to carry out supply chain management.