Abstract: Supply chain finance provides new ideas for the problem of financing difficulties for agriculture-related enterprises. In this regard, the article selected agricultural enterprises listed in China’ s A-shares from 2014 to 2024 as research samples, and adopted the fixed effect model to conduct an empirical analysis on the correlation between supply chain finance and financing constraints. It also examined the mediating role of credit risk and information asymmetry in their relationship and further analyzed the impact of differences in supply chain concentration and industry background. The research found that supply chain finance had a significant improvement effect on the financing difficulties of agriculture-related enterprises and could effectively alleviate their financing pressure. Moreover, by reducing the level of credit risk and the degree of information asymmetry, supply chain finance could further enhance its effectiveness in alleviating the financing predicament of agricultural enterprises. A deeper analysis revealed that in agricultural enterprises with stable supply chain structures and within the manufacturing industry, supply chain finance showed more significant effects in easing financing constraints. This article expands the research dimension of financing constraints for agricultural enterprises and provides new theoretical basis for different types of enterprises to carry out supply chain management.