Abstract: As one of the new agricultural management entities, agricultural industrialization leading enterprises play an irreplaceable role in promoting comprehensive rural revitalization and the modernization of agriculture and rural areas. This article focused on the provincial-level leading enterprises in Yunnan’ s agricultural industrialization, and used methods such as spatial analysis and econometric analysis to study the influencing factors of the spatial distribution of leading enterprises in agricultural industrialization. This study found that, the spatial distribution of the leading enterprises in Yunnan’ s agricultural industrialization was uneven, and more obvious hot spots had formed in areas such as Kunming, Qujing, Chuxiong, Yuxi and Baoshan, with relatively few and scattered cold spots. The total output value of agriculture, forestry, animal husbandry and fishery, the mileage of highways, the total retail sales of consumer goods, urbanization rate, and the number of rural employees were positively correlated with the number of leading enterprises in agricultural industrialization, while the total amount of import and export trade was negatively correlated with the number of leading enterprises. Based on this situation, strategies and suggestions for the spatial layout, cultivation and development of the leading enterprises in Yunnan’ s agricultural industrialization were proposed.