• Chinese Science Citation Database (CSCD) Source Journals
  • A Guide to the Core Journals of China
  • Top 100 Sci-Tech Journals of Chinese Universities
  • Chinese Science and Technical Core Journals
  • China Agriculture and Forestry Core Journals (Category A)
Advanced Search
BAI Bo, ZHENG Yaqiang, LIU Siyu, et al. Pathogenicity of Metarihizium anisopliae and Beauveria bassiana against the 2nd Instar Larva of Hololtrichia diomphalia Batesa[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2016, 31(1): 30-34. DOI: 10.16211/j.issn.1004-390X(n).2016.01.005
Citation: BAI Bo, ZHENG Yaqiang, LIU Siyu, et al. Pathogenicity of Metarihizium anisopliae and Beauveria bassiana against the 2nd Instar Larva of Hololtrichia diomphalia Batesa[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2016, 31(1): 30-34. DOI: 10.16211/j.issn.1004-390X(n).2016.01.005
shu

Pathogenicity of Metarihizium anisopliae and Beauveria bassiana against the 2nd Instar Larva of Hololtrichia diomphalia Batesa

  • 1.

    College of Plant Protection, Microorganism Fermentation Engineering Research Center, Yunnan Agricultural University, Kunming 650201, China

  • 2.

    College of Agronomy and Bioscience, Yunnan Agricultural University, Kunming 650201, China

More Information
  • Received Date: October 06, 2014
  • Revised Date: January 13, 2015
  • Published Date: January 15, 2016
    Graphical Abstract
    • Abstract
  • In order to screen higher virulent entomopathogenic fungus for the biocontrol of Holotrichia diomphalia Batesa, the virulence and effect on the protein content in the blood-lymph of the 2nd instar larva of H. diomphalia after inoculation of three entomopathogenic fungus KMa0107 of Metarihizium anisopliae,strain Bb070610 and Bb070917 of Beauveria bassiana (Balsamo) Vuillemin was tested in the laboratory. Results showed that the lethal dosage caused by KMa0107,Bb070610 and Bb070917 was significantly different, and the lethal time was also significantly different(F＝19.45,P0.01). The logarithmic value of LC50 was (9.451.71)~(4.730.37),(9.761.87)~(5.200.79),(10.481.24)~(5.990.43) for strain KMa0107,Bb070610 and Bb070917,respectively. The LT50 ranged 5.7~8.7d,6.3~8.6d,6.4~9.2d and for H. diomphalia at the concentration of 105~108 spores/mL. The content of the protein in the haemocytes decreased 4 d after inoculation, significantly lower than in the control, and the protein content in blood-lymph in the 2nd instar larvae after inoculation of strain KMa0107,Bb070610 was significantly lower than in the larvae after inoculation of strain Bb070917. Overall,strain KMa0107 of M.anisopliae and Bb070610 of B.bassiana showed high pathogenicity and with biocontrol potentiality against 2nd instar larva of H.diomphalia.
    • FullText(HTML)
    • References (19)
  • [1] 张继祖,徐金汉.中国南方地下害虫及其天敌[M].北京:中国农业出版社,1996.
    [2] [2] 魏鸿均,黄文琴. 中国地下害虫研究概述[J]. 昆虫知识,1992,29(3):168.
    [3] [3] LACEY L A,FRUTOS R,KAYA H K,et al. Insect pathogens as biological control agents: do they have a future? [J]. Biological Control,2001,21(3):230.
    [4] [4] VEGA F E,MEYLING N V,LUANGSARD J J,et al. Fungal entomopathogens[M] // Insect Pathology. 2nd ed. San Diego,CA:Academic Press, 2012: 171.
    [5] [5] WANG C S,FENG M G. Advances in fundamental and applied studies in China of fungal biocontrol agents for use against arthropod pests[J]. Biological Control,2014,68 (1) :129.
    [6] [6] 黄基荣,谢佩华,姚丽娟. 绿僵菌对金龟子的感病性试验[J]. 生物防治通报, 1994,10(4):175.
    [7] [7] 陈祝安,黄基荣. 不同来源绿僵菌对云斑鳃金龟的致病力评价[J]. 微生物学通报,1997,24(2):81.
    [8] [8] MILNER R J,SAMSON P,MORTON R. Persistence of conidia of Metarhizium anisopliae in sugarcane fields: effect of isolate and formulation on persistence over 3-5 years[J]. Biocontrol Science and Technology,2003,13(5):507.
    [9] [9] 陈斌,邓裕亮,李正跃,等. 绿僵菌对小云斑鳃金龟毒力及在土壤中的宿存[J]. 西南农业大学学报(自然科学版),2005,26(5):580.
    [10] [10] 张丽坤,李国勋,文景芝,等.球孢白僵菌对东北大黑鳃金龟(Coleoptera:Melolonthidae)的侵染效果[J]. 东北农业大学学报,1997,28(3):20.
    [11] [11] 贾春生. 绿僵菌对东北大黑鳃金龟的时间-剂量-死亡率模型分析[J]. 中国植保导刊,2005,25(10):11.
    [12] [12] 李建武. 生物化学实验原理和方法[M]. 北京:北京大学出版社,2002.
    [13] [13] 陈斌,李正跃, 和淑琪. 金龟子绿僵菌KMa0107菌株对三种玛绢金龟幼虫的致病力[J].中国生物防治,2010,26(1):18.
    [14] [14] 唐启义,冯明光. 实用统计分析及其DPS 数据处理系统[M]. 北京:科学出版社,2002.
    [15] [15] 农向群,张爱文,邓春生. 卵孢白僵菌优良菌株的筛选和选育[J]. 生物防治通报,1994,10(1):22.
    [16] [16] 李耀发,高占林,党志红,等.18种杀虫剂对华北大黑鳃金龟和铜绿丽金龟的毒力比较[J].植物保护科学,2008,24(3):296.
    [17] [17] 刘艳涛,席国成,冯晓洁,等.不同龄期大黑鳃金龟幼虫对两种剂型杀虫剂的敏感性测定[J].北方园艺,2013(11):113.
    [18] [18] 蒲蜇龙,李增智.昆虫真菌学[M].合肥:安徽科学技术出版社,1996.
    [19] [19] HUXHAM I M, LACKIE A M, MCCORKINDALE N J. Inhibitory effects of cyclodepsipeptides, destruxins,from the fungus Metarhizium anisophae,on celular immunity in insects[J]. Journal of Insect Physiology,1989,35(2):97.
    • Related Articles

  • Related Articles

    [1]Fenglei DING, Le ZHANG, Lei YU, Hejuan WANG, Lili ZHANG, Xiaoxiao LI, He SONG, Zhaorong DONG. Differences in Nitrogen Accumulation and Root Zone Soil Nitrogen Transformation in Different Maize Genotypes[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2022, 37(6): 1004-1013. DOI: 10.12101/j.issn.1004-390X(n).202111072
    [2]Fupeng MEI, Zhiyuan HUANG, Jiaxiang WU, Mengzhuang CUN, Guiru ZHAO, Shihua LI, Wangfu YANG, Shuhui ZI. Effects of Nitrogen Reduction on Intercropping Advantage of Maize and Aconitum vilmorinianum[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2022, 37(5): 890-896. DOI: 10.12101/j.issn.1004-390X(n).202111008
    [3]Yunling CUI, Liqin ZHANG. Effect of SODmN Reduction Application on the Yield, Profit and Nutrient Utilization of Spring Maize in Hexi Oasis Irrigation Area[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2021, 36(2): 353-358. DOI: 10.12101/j.issn.1004-390X(n).201907056
    [4]Zhiying GAO, Zhaopei ZHANG, Lei FAN, Yonghui YANG. Effects of Light Intensity and Nitrogen Level on the Growth, Photosynthetic Physiology and Dry Matter Accumulation of Maize[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2020, 35(2): 187-195. DOI: 10.12101/j.issn.1004-390X(n).201811001
    [5]Tongxin AN, Yuanman YANG, Feng ZHOU, Zhiwei FAN, Mengli CHEN, Jing LU, Bozhi WU. Effect of Maize and Potato Intercropping on Their Root Growth and Distribution[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2018, 33(2): 363-370. DOI: 10.12101/j.issn.1004-390X(n).201504030
    [6]XU Zhaoli, YANG Yuhong, DUAN Yuqi, JIN Yan. Effect of Nitrogen Application Rate and Nitrate Nitrogen Ratio on Accumulation and Distribution of P, K and Cl of Flue-cured Tobacco[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2017, 32(6): 1028-1035. DOI: 10.16211/j.issn.1004-390X(n).2017.06.010
    [7]JIA Manman, XIAO Jingxiu, TANG Li, ZHENG Yi. Effects of Nitrogen Supply on Yields and Photosynthesis Characteristics of Crops in Wheat and Broad Bean Intercropping[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2017, 32(2): 350-357. DOI: 10.16211/j.issn.1004-390X(n).2017.02.022
    [8]ZHANG Leichang, TANG Li, DONG Yan, ZHENG Yi. Nitrogen Absorption of Crops Affected by Root Interaction in Maize and Soybean Intercropping[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2016, 31(6): 1111-1119. DOI: 10.16211/j.issn.1004-390X(n).2016.06.022
    [9]QIN Xiaomin, ZHENG Yi, TANG Li, LONG Guangqiang. Effects of Nitrogen Application Rates on Rhizosphere Soil Enzyme Activity and Potential Nitrification in Maize and Potato Intercropping[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2015, 30(6): 886-894. DOI: 10.16211/j.issn.1004-390X(n).2015.06.010
    [10]SUN Jianping, TANG Li, XU Yongbo, ZHENG Yi. Effect of Nitrogen Application on Urease Activity in Rhizosphere Soil of Wheat and Faba Bean Intercropping System[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2015, 30(3): 464-470. DOI: 10.16211/j.issn.1004-390X(n).2015.03.023

  • Cited by

    Periodical cited type(10)

    1. 阮贞静，周静，李海，王强凤，傅敬东，纪韵祚，张振林，安曈昕. 氮肥追施对烤烟套作早熟玉米氮素利用及产质量的影响. 云南农业大学学报(自然科学). 2024(06): 151-159 .
    2. 常青，李立军，渠佳慧，张艳丽，韩冬雨，赵鑫瑶. 土默川平原麦后复种饲用玉米‖油菜模式的增产优势及氮素利用效率. 作物杂志. 2023(03): 167-174 .
    3. 梅富鹏，黄之远，吴加香，寸梦壵，赵桂茹，李世华，杨旺福，字淑慧. 减氮对玉米黄草乌间作优势的影响. 云南农业大学学报(自然科学). 2022(05): 890-896 .
    4. 郭涛，邢英英，姜文婷，李卓远，张腾，密菲瑶，王秀康. 滴灌施肥对马铃薯根层土壤养分含量、产量及水分利用效率的影响. 河南农业科学. 2021(09): 16-24 .
    5. 乔冰洁，杨小华，吴静，徐宝连. 马铃薯-玉米间作模式研究进展. 中国马铃薯. 2021(05): 463-468 .
    6. 赵薇，伊文博，王顶，吴开贤，赵平，龙光强，汤利. 间作对马铃薯种植土壤硝化作用和硝态氮供应的影响. 应用生态学报. 2020(12): 4171-4179 .
    7. 钱韩玲，朱启林，周龙，龙光强，汤利. 间作促进作物磷吸收的氮素调控效应. 农业资源与环境学报. 2019(04): 471-479 .
    8. 王雪蓉，张润芝，李淑敏，许宁，牟尧，张春怡. 不同供氮水平下玉米/大豆间作体系干物质积累和氮素吸收动态模拟. 中国生态农业学报(中英文). 2019(09): 1354-1363 .
    9. 钱欣，许和水，葛军勇，曾昭海，任长忠，郭来春，王春龙，胡跃高. 施氮量及间作对燕麦、向日葵生产力及土壤硝态氮累积的影响. 中国农业大学学报. 2018(03): 1-9 .
    10. 刘鸿高，李建宾，顾才上，吴伯志. 间作马铃薯覆盖不同地膜对土壤温湿度和产量的影响. 云南农业大学学报(自然科学). 2017(01): 44-51 .

    Other cited types(7)

Catalog

    Get Citation
    PDF
    XML
    Article views (2063) PDF downloads (603) Cited by(17)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles
    • Copyright © Journal of Yunnan Agricultural University Editorial Department
    • Address: Room 108, Adult Education Building, Yunnan Agricultural University, No. 95 Heijin Road, Kunming, Yunnan Postal Code: 650201
    • Tel: +86-871-65227711 E-mail: ynauzkxb@foxmail.com

    Supported by: Beijing Renhe Information Technology Co., Ltd.

    Return