Cloning, Bioinformatics Analysis and Expression Dynamics of LoXTH23 Gene in Bulb Dormancy Release Process of Lily

Purpose To study the role of LoXTH23 gene in the process of dormancy release in Lilium oriental ‘Siberian’.

Methods The bulbs of L. oriental ‘Siberian’ were used as test materials, and a XTH family gene with significant differences in dormancy release period was screened out from the results of pre-transcriptome sequencing, which amplified the full-length sequence of the cDNA by RT-PCR, named LoXTH23. Bioinformatics software was used to analyse the structure, physicochemical properties, etc. of the LoXTH23 gene, and fluorescence quantitative PCR was used to determine the relative expression level of the LoXTH23 gene in the process of bulb dormancy release.

Results The open reading frame of LoXTH23 gene was 858 bp in length, encoding 285 amino acids, including GH16 structural domain, and belonging to the XTH family of genes; the relative molecular weight of the encoded protein was about 3.19 ku, and the theoretical isoelectric point was 6.19; it was an unstable hydrophilic protein with the presence of transmembrane structural domains and a signal peptide; random coiling was dominant in the secondary structure, and there were 30 phosphorylation sites. The results of fluorescence quantitative PCR showed that the expression level of LoXTH23 gene was the highest at 10 days, when the bulb dormancy was completely released.

Conclusion LoXTH23 may play an important role in the regulation of lily bulb dormancy release.