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ZHANG Jimin, WANG Hao, JIA Wenjie, et al. Cloning, Bioinformatics Analysis and Expression Dynamics of LoXTH23 Gene in Bulb Dormancy Release Process of Lily[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2023, 38(6): 1041-1048. DOI: 10.12101/j.issn.1004-390X(n).202303001
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To study the role of LoXTH23 gene in the process of dormancy release in Lilium oriental ‘Siberian’.
The bulbs of L. oriental ‘Siberian’ were used as test materials, and a XTH family gene with significant differences in dormancy release period was screened out from the results of pre-transcriptome sequencing, which amplified the full-length sequence of the cDNA by RT-PCR, named LoXTH23. Bioinformatics software was used to analyse the structure, physicochemical properties, etc. of the LoXTH23 gene, and fluorescence quantitative PCR was used to determine the relative expression level of the LoXTH23 gene in the process of bulb dormancy release.
The open reading frame of LoXTH23 gene was 858 bp in length, encoding 285 amino acids, including GH16 structural domain, and belonging to the XTH family of genes; the relative molecular weight of the encoded protein was about 3.19 ku, and the theoretical isoelectric point was 6.19; it was an unstable hydrophilic protein with the presence of transmembrane structural domains and a signal peptide; random coiling was dominant in the secondary structure, and there were 30 phosphorylation sites. The results of fluorescence quantitative PCR showed that the expression level of LoXTH23 gene was the highest at 10 days, when the bulb dormancy was completely released.
LoXTH23 may play an important role in the regulation of lily bulb dormancy release.
| [1] |
FARKAS V, SULOVA Z, STRATILOVA E, et al. Cleavage of xyloglucan by nasturtium seed xyloglucanase and transglycosylation to xyloglucan subunit oligosaccharides[J]. Archives of Biochemistry and Biophysics, 1992, 298(2): 365. DOI: 10.1016/0003-9861(92)90 423-T.
|
| [2] |
FRY S C, SMITH R C, RENWICK K F, et al. Xyloglucan endotransglycosylase, a new wall-loosening enzyme activity from plants[J]. Biochemical Journal, 1992, 282(3): 828. DOI: 10.1042/bj2820821.
|
| [3] |
NISHITANI K, TOMINAGA R. Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule[J]. Journal of Biological Chemistry, 1992, 267(29): 21064. DOI: 10.1016/S0021-9258(19)367 97-3.
|
| [4] |
COSGROVE D J. Growth of the plant cell wall[J]. Nature Reviews Molecular Cell Biology, 2005, 6(11): 861. DOI: 10.1038/nrm1746.
|
| [5] |
BAUMANN M J, EKLOF J M, MICHEL G, et al. Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: biological implications for cell wall metabolism[J]. The Plant Cell, 2007, 19(6): 1963. DOI: 10.1105/tpc.107.051391.
|
| [6] |
SALADIÉ M, ROSE J K C, COSGROVE D J, et al. Characterization of a new xyloglucan endotransglucosylase/hydrolase (XTH) from ripening tomato fruit and implications for the diverse modes of enzymic action[J]. The Plant Journal, 2006, 47(2): 282. DOI: 10.1111/j.13 65-313X.2006.02784.x.
|
| [7] |
KALLAS A M, PIENS K, DENMAN S E, et al. Enzymatic properties of native and deglycosylated hybrid aspen ( Populus tremula× tremuloides) xyloglucan endotransglycosylase 16A expressed in Pichia pastoris[J]. Biochemical Journal, 2005, 390(1): 105. DOI: 10.1042/BJ20041749.
|
| [8] |
VAN SANDT V S T, GUISEZ Y, VERBELEN J P, et al. Analysis of a xyloglucan endotransglycosylase/hydrolase (XTH) from the lycopodiophyte Selaginella kraussiana suggests that XTH sequence characteristics and function are highly conserved during the evolution of vascular plants[J]. Journal of Experimental Botany, 2006, 57(12): 2922. DOI: 10.1093/jxb/erl064.
|
| [9] |
LIU Y B, LU S M, ZHANG J F, et al. A xyloglucanendotransglucosylase/hydrolase involves in growth of primary root and alters the deposition of cellulose in Arabidopsis[J]. Planta, 2007, 226(6): 1560. DOI: 10.1007/s00 425-007-0591-2.
|
| [10] |
SASIDHARAN R, CHINNAPPA C C, STAAL M, et al. Light quality-mediated petiole elongation in Arabidopsis during shade avoidance involves cell wall modification by xyloglucan endotransglucosylase/hydrolases[J]. Plant Physiology, 2010, 154(2): 990. DOI: 10.1104/pp.110.16 2057.
|
| [11] |
SILVIA R, TERESA J, EMILIA L, et al. The gene for a xyloglucan endotransglucosylase/hydrolase from Cicer arietinum is strongly expressed in elongating tissues[J]. Plant Physiology and Biochemistry, 2005, 43(2): 169. DOI: 10.1016/j.plaphy.2005.01.014.
|
| [12] |
张亚楠, 王婷, 欧斯艳, 等. 大豆响应低磷胁迫的 XTH基因鉴定及XTH38调节根系生长研究[J]. 植物营养与肥料报, 2022, 28(7): 1181. DOI: 10.11674/zwyf.2021 562.
|
| [13] |
HE H, RACHID S, YANG Q. Changes in OsXTH gene expression, ABA content, and peduncle elongation in rice subjected to drought at the reproductive stage[J]. Acta Physiologiae Plantarum, 2009, 31(4): 749. DOI: 10.1007/s11738-009-0287-2.
|
| [14] |
GENOVESI V, FORNALÉ S, FRY S C, et al. ZmXTH1, a new xyloglucan endotransglucosylase/hydrolase in maize, affects cell wall structure and composition in Arabidopsis thaliana[J]. Journal of Experimental Botany, 2008, 59(4): 875. DOI: 10.1093/jxb/ern013.
|
| [15] |
CHO S K, KIM J E, PARK J A, et al. Constitutive expression of abiotic stress-inducible hot pepper CaXTH3, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants[J]. FEBS Letters, 2006, 580(13): 3136. DOI: 10.1016/j.febslet.2006.04.062.
|
| [16] |
PAN C L, YAO L X, YU L Y, et al. Transcriptome and proteome analyses reveal the potential mechanism of seed dormancy release in Amomum tsaoko during warm stratification[J]. BMC Genomics, 2023, 24(1): 99. DOI: 10.1186/S12864-023-09202-X.
|
| [17] |
MA X W, CHEN Y, LIU M Y, et al. Genome-wide analysis of the XTH gene family and functional analysis of DlXTH23.5/25 during early longan somatic embryogenesis[J]. Frontiers in Plant Science, 2022, 13: 1043464. DOI: 10.3389/fpls.2022.1043464.
|
| [18] |
王浩, 孙亮, 贾文杰, 等. 百合AP2/ERF家族转录因子基因 LoERF4的克隆和生物信息学分析[J]. 云南农业大学学报(自然科学), 2022, 37(6): 1030. DOI: 10.12101/j.issn. 1004-390X(n).202111078.
|
| [19] |
梁蕤, 徐雷锋, 毕蒙蒙, 等. 柠檬色百合实时荧光定量PCR内参基因筛选[J]. 中国农业大学学报, 2023, 28(2): 73. DOI: 10.11841/j.issn.1007-4333.2023.02.06.
|
| [20] |
HAYASHI T, KAIDA R. Functions of xyloglucan in plant cells[J]. Molecular Plant, 2011, 4(1): 24. DOI: 10.10 93/mp/ssq063.
|
| [21] |
EDELMANN H G, FRY S C. Effect of cellulose synthesis inhibition on growth and the integration of xyloglucan into pea internode cell walls[J]. Plant Physiology, 1992, 100(2): 993. DOI: 10.1104/pp.100.2.993.
|
| [22] |
CAVALIER D M, LEROUXEL O, NEUMETZLER L, et al. Disrupting two Arabidopsis thaliana xylosyltransferase genes results in plants deficient in xyloglucan, a major primary cell wall component[J]. The Plant Cell, 2008, 20(6): 1519. DOI: 10.1105/tpc.108.059873.
|
| [23] |
VISSENBERG K, FRY S C, PAULY M, et al. XTH acts at the microfibril-matrix interface during cell elongation[J]. Journal of Experimental Botany, 2005, 56(412): 673. DOI: 10.1093/jxb/eri048.
|
| [24] |
陈龙, 张沿政, 李永光, 等. GmXTH23基因的克隆及抗旱性鉴定[J]. 江西农业大学学报, 2020, 42(5): 905. DOI: 10.13836/j.jjau.2020101.
|
| [25] |
MIRANTI C K, BRUGGE J S. Sensing the environment: a historical perspective on integrin signal transduction[J]. Nature Cell Biology, 2002, 4(4): E83. DOI: 10.1038/ncb0 402-e83.
|
| [26] |
İŞKIL R, SURGUN-ACAR Y. Expression analysis of cell wall assembly and remodelling-related genes in Arabidopsis roots subjected to boron stress and brassinosteroid at different developmental stages[J]. Acta Botanica Brasilica, 2018, 32(7): 1. DOI: 10.1590/0102-33062018 abb0023.
|
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