Bioinformatics Analysis of PAD1 Gene of Saccharomyces cerevisiae

PurposeTo study the function of the Saccharomyces cerevisiae PAD1 gene, laying the foundation for studying the metabolic processes and pathways of aromatic compounds in S. cerevisiae.

MethodsPAD1 gene of S. cerevisiae was electronically cloned, and then the gene analyzed and predicted by bioinformatics analysis.

ResultsThe S. cerevisiae PAD1 gene was located on chromosome Ⅳ 1510902-1511630, with a total length of 729 bp, it could encode 242 amino acids. Phylogenetic tree analysis showed that the protein encoded by this gene was the most similar to phenylacrylic acid decarboxylase (AAA20484.1), and the homology was up to 99.59%. The chemical formula of the protein was C₁₂₀₈H₁₉₅₀N₃₂₈O₃₃₆S₉, and the molecular weight was about 26 733.31 ku. It was a stable hydrophobic protein and played a role in mitochondria. The secondary structure prediction results did not find any obvious coiled helix region, but some transmembrane regions existed, and the secondary structural components were mainly α-helix and random coil, extended strand and β-bend were scattered in the protein.

ConclusionThe S. cerevisiae PAD1 gene, which encodes an aromatic carboxylic acid decarboxylase, encodes a structurally stable protein, and it may be involved in metabolism in the mitochondria and it is directly linked to the metabolism of aromatic carboxylic acids and their tolerance to aromatic carboxylic acids in S. cerevisiae.