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保山猪PCSK4的转录调控网络构建及生物信息学分析
分析保山猪蛋白转化酶枯草素/酮蛋白4型(proprotein convertase subtilisin/kexin type 4，PCSK4)的分子特征、转录表达调控和蛋白功能。
基于保山猪睾丸全转录组数据，通过生物信息学方法对PCSK4进行功能注释，并构建PCSK4的长链非编码RNA和小RNA (miRNA)调控网络。
PCSK4编码序列长2 505 bp，编码834个氨基酸。蛋白质功能分析表明：PCSK4具有较多无规则卷曲和磷酸化位点。进化及邻近基因分析表明：PCSK4在进化过程中保守性较高。功能富集分析表明PCSK4与多个参与精子获能、受精、精卵识别的蛋白(如ACRBP、ACR、ADAM2等)存在互作关系，这些蛋白对应基因的表达量表明：PCSK4与MBTPS1、ACRBP、CDKN2A、DPY19L2显著相关。竞争性内源RNA 调控网络分析表明5个miRNA可靶向调控PCSK4，功能注释显示其在生殖过程、蛋白质加工、受精等功能中发挥重要作用。
本研究为进一步探讨PCSK4在保山猪精子发生过程，特别是受精与精子成熟阶段等重要生物学过程提供了基础资料。
Transcriptional Regulatory Network Construction and Bioinformatics Analysis of PCSK4 in Baoshan Pig
To analyze the molecular characteristics, transcriptional expression regulation and protein function of proprotein convertase subtilisin/kexin type 4 (PCSK4) in Baoshan pig.
Based on the full transcriptome data of Baoshan pig testis, the function of PCSK4 was annotated by bioinformatics methods, and the long non-coding RNA and miRNA regulatory network of PCSK4 was constructed.
The length of PCSK4 coding sequence was 2 505 bp, encoding 834 amino acids. Protein functional analysis showed that PCSK4 had more random coils and phosphorylation sites. Evolution and neighboring gene analysis showed that PCSK4 was highly conserved in evolution process. Functional enrichment analysis showed that PCSK4 interacted with several proteins (ACRBP, ACR, ADAM2, etc.) involved in the processes such as capacitation, fertilization, and gamete recognition. The expression levels of the corresponding genes of these proteins showed that PCSK4 was significantly correlated with MBTPS1, ACRBP, CDKN2A and DPY19L2. Competitive endogenous RNA regulatory network analysis showed that there were five miRNAs could target and regulate PCSK4, and functional annotation showed that it played an role in reproductive processes, protein processing, fertilization and other functions.
This study provides basic data for further study on the important biological processes of PCSK4 in Baoshan pig spermatogenesis, especially fertilization and sperm maturation.
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Keywords:
- Baoshan pig /
- PCSK4 /
- bioinformatics analysis /
- protein-protein interaction /
- functional annotation
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