Determination of Three Phenylalanine Components in Sinodielsia yunnanensis by HPLC

Purpose In order to compare the content of ferulic acid, caffeic acid and chlorogenic acid in Sinodielsia yunnanensis samples from two main origins, we established HPLC analysis method of three phenylalanine components.Method We used Waters SunFire C₁₈ (150 mm×4.6 mm,3.5 μm) column with gradient elution consisting of methanol and 0.1% formic acid in water at a flow rate of 1 mL/min. The column temperature was set at 30℃. The detection wavelength was 350 nm. The injection volume was 10 μL.Result The standard curve of ferulic acid, caffeic acid, and chlorogenic acid respectively showed a good linearity (r> 0.999 0) in the concentration range of 0.102-51, 0.108-54, 0.112-56 μg/mL, and the average recoveries were 98.15%, 100.48%, 101.25%, RSD were 1.75%, 1.69%, 1.23%.Conclusion This method was used to comparing content detection of S.yunnanensis of Tibet and S.yunnanensis of Yunnan, the content of three components in Tibet celery were all lower than those in Yunnan celery. The method is simple, accurate, reliable and reproducible, and can be used to do quantitative analysis of the three phenylalanine components in S. yunnanensis herbs.