Cloning of BmCecropinB6 Antibacterial Peptide Gene and Auto-induction Expressing of Recombinant Proteins from Bombyx mori

Purpose Bombyx mori CecropinB6(BmCecropinB6) is an important antibacterial peptide of silkworm immune system, it has strong antibacterial function on against gram positive and gram negative bacteria,but auto-induction expressing of recombinant protein has been reported.Method In this study, the total RNA as a template was extracted from the silkworm midgut tissue, a pairs of specific primer was designed, then B. CecropinB6 (BmCecropinB6) cDNA fragment was cloned by RT-PCR technology. pET32a-BmCecropinB6 prokaryotic expression vector was constructed, BmCecropinB6 recombinant protein was expressed by auto-induction expressing system, and expressing yields was further analyzed.Result The BmCecropinB6 gene is 192 bp nucleotides in sizes, and encodes 63 amino acids, the auto-induction expressing of recombinant BmCecropinB6 protein is about 27 ku, the expressing yields is 41%.Conclusion The research establish a basis for further application of BmCecropinB6 antimicrobial peptides.