Establishment of Micropropagation System from Root Tip Segment of Clematis apiifoliavar

Using root tip of Clematis apiifoliavar as explant, the effects of basic medium, culturing conditions,hormones and concentrations of antioxidants media on the induction of adventitious buds were investigated. The result showed that the best regeneration medium was Murashige and Skoog (MS) basal medium with Gamborg B5 vitamins supplemented with 2.0 mg/L 6-benzyladenine (BA), 0.5 mg/L naphthalenacetic acid (NAA).More than 78.5% root tip segments produced adventitious shoots, with a mean number of adventitious shoots per explant of 8.9.The optimum growing medium was observed on improved MS medium supplemented with 2.0 mg/L BA and 1.0 mg/L NAA. Over 69.4% regeneration plantlets were able to root after transferred to the modified MS medium supplemented with 1.0 mg/L indole-3-butyric acid, 0.5 mg/L napthaleneacetic acid and 20 g/L sucrose. When micropropagated plantlets with well-developed root systems were transferred to planting bed containing a mixture of perlite, vermiculite and humus (4:1:1,volume ratio) in a greenhouse,93.5%of the plantlets survived.This regeneration system using root tips of C. apiifoliavar provides a foundation for large-scale vegetative propagation.