Basic Research on Bletilla Based on ITS2 Barcode

In this study, we used the ITS2 barcode to identify the distribution of indigenous species of Bletilla striata in Yunnan, analyzed the officinal species and indigenous species. Materials were gathered from the southeast, northeast, northwest and western region of Yunnan. Then the genomic DNA was isolated, ITS2 sequence was amplified and sequenced. Analytic identifications were performed by MEGA 5.1, then predicted the secondary structure of ITS2 sequences.The results showed that a significant difference was observed between the ITS2 sequence of B. striata and indigenous species, they were singly divided into two classes in the NJ tree. The study showed that the ITS2 barcode could differentiate medicinal material and indigenous species of B. striata, and could be used as a potential DNA barcode to preliminarily analyze the medicinal plants B. striata and its adulterants.