Analysis of Genetic Diversity in Leucaena Germplasm Using Stylosanthes EST-SSRs

In order to analyze the genetic diversity of 48 Leucaena germplasm resources, 20 more polymorphic effective markers were screened from 184 Stylosanthes EST-SSR markers. The result showed that 119 of 184 Styloanthes EST-SSR markers were effective markers and 55 markers were polymorphic, the effective and polymorphic transferable rate was 64.67% and 29.89%, respectively. Furthermore, in the genetic diversity analysis and cluster analysis of 48 Leucaena germplasm resources, the range of GS is 0.618 2~0.941 2, and the average of PIC and Shannon index are 0.266 and 0.536, respectively. All accessions were divided into 5 major group(I,II,III,IV and V). All of the Leucaena diversifolia germplasm were clustered in Group I, and Leucaena leucaephala accessions from different sites were divided into Group V. Accession c27 clustered separately in Group II is a good material for crossbreeding in Leucaena. In summary, the Stylosanthes EST-SSR markers can be utilized in analysis of genetic diversity, mining and identification of gene and breeding in Leucaena. Due to the complex genetic relationships, analysis of genetic relationships of Leucaena germplasm should be researched generally with several tools.