The Tissue Culture Research with Burgeon of Psammosilene tunicoides

With MS as the basic culture medium, appended plant growth regulator with different concentrations, the new germination and axillary Psammosilene tunicoides stem section were inoculated in the culture medium, to select the best rapid propagation project of the tissue culture of P.tunicoides. The results showed that:the optimal disinfection combination of P.tunicoides explants was 75% ethanol continuous 0.5 min and 0.1% HgCl2 for 7 min, pollution rate reduced to 35% and survival rate can be as high as 73%. The optimal combination of differentiation culture of P.tunicoides was MS+0.01 mg/L TDZ+0.5mg/L NAA, induction rate could reach 90%. The better proliferation medium of multiple bud clumps was MS+0.05 mg/L 6-BA+0.5 mg/L NAA, the biggest proliferation of seedlings reached 22.3 times. The most suitable rooting culture was 1/2 MS+0.15 mg/L NAA+0.1 mg/L IBA, rooting rate reached 95% and the root growth in good condition. The results provided theoretical and practical guidance for P.tunicoides, including rapid expansion of cultivation, conservation of germplasm resources and genetic improvement.