Genetic Diversity among Dendrobium with Known Origins Based on the ISSR and AFLP Markers

The genetic diversity of 51 Dendrobium materials (including 35 D. officinale germplasm resources) was studied by ISSR and AFLP. Six primers chosen from ISSR markers, totally generated 79 amplification bands, on average every primer generate 13.17 amplification bands, and the ratio of polymorphism bands reached 100%. Five primer pairs by AFLP markers, generated altogether 207 bands, 206 of which were polymorphic bands and the average PPB was 99.52%. The results of two molecular markers showed that the level of differentiation between groups was larger, differentiation of groups was comparatively large and gene flow between groups was smaller. The cluster analysis indicated that ISSR and AFLP marker technology could effectively separate the materials, and basically reflect the genetic relationship. Two markers could be applied to detecting genetic diversity analysis of plants in Dendrobium.