The Key Factors Effecting on Rapid Propagation of Asparagus officinalis

The purpose of this paper is to study the key factors effecting on sterile culture establishment, proliferation and rooting for asparagus A001 with early-ripe, high yield, sturdy, high resistance and tolerance to low temperature, which was selected from the clone variation of Italy asparagus. The results showed that:(1) the effects of disinfection could reach above 70% when the shoot tip explants were treated with 2% NaClO solution for 8min and the stem explants were treated with 2% NaClO solution for 12min after treated 30 s with 75% ethanol; (2) MS+NAA 0.3mg/L+BA 0.2mg/L+KT 0.2mg/L was the best proliferation culture medium, and the proliferation coefficient was up to 4.4; (3) 1/2 MS+NAA 0.3mg/L+KT 0.4mg/L+ancymidol 1.0mg/L was the best medium for rooting, and rooting rate reached 72%. According to the results from single factor, it was found that ancymidol played a significant role improving the rooting of asparagus; high concentration NAA and BA tended to cause vitrification of the asparagus; adding KT and ancymidol with suitable concentration can reduce the vitrification effectively. In rapid propagation of asparagus, NAA 0.5mg/L would inhibit asparagus growth obviously.