A Study on in vitro Rapid Propagation Technology for Calotropis gigantea

The tender apical buds of Calotropis gigantea was used as explants to study the effects of 0.1% HgCl2 at different sterilization times on the livability of the sterile apical buds, and taking the stem segments of aseptic buds as materials, and was cultured on MS culture media with different concentrations of 6-BA, TDZ, NAA, IBA. The result showed that 0.1% HgCl2 sterilization for 10 minutes was the optimal and livability of the sterile apical buds was 82.2%. The rapid propagation medium was MS+6-BA 1.0mg/L+NAA 0.1mg/L, where the propagation coefficient was up to 3.9 with 25 days. The optimal medium for rooting was 1/2MS+IBA 0.1mg/L, and resulted in 100% rooting rate. The survival rate of rooted plantlets was recorded as 90% after 45 days when plantlets were transferred in pots containing mixed substrateV(red subsoil):V (humus soil):V (perlite)=2:2:1). The tissue culture and rapid proliferation system was successfully established, and it could provide important theoretical basis and practical guidance for rapid proliferation of superior individuals and its genetic improvement.