Connection on Tobacco Seed Vitality and Budding in Germplasm Resources Bank

In order to propose the renew index of budding for tobacco germplasm resources, the emergence rates of 63 tobacco seeds whose budding rates were under 50% and stored for 3~30 years and 8 tobacco seeds whose budding rates were 55%~95% and stored for 4~24 years were detected in the man-made climate controller, and the seed budding characteristics were studied according to the different germination energy subsection. The results showed that the emergence rate rose with the increasing of seed germination energy and germination percentage. The average emergence rates in the 7th day and 14th day were lower than their germination energy and germination percentages, even if delaying budding in the 70th day. With the increasing of seed germination energy and germination percentage, the emergence rate also rose according to its total subsection. When the seed germination energy of subsections was from 20.1% to 30.0%, the emergence rate could reach 100% in 14th day. When the seed germination energy of subsections was higher than 30.1%, the emergence rate could reach 100% in 7th day. The correlation analysis result showed that, the storing years had negative correlation with the seed germination energy, germination percentage and emergence rate in 7th day, while there was positive correlation between the seed germination energy and germination percentage, the seed germination energy and germination percentage had significantly positive correlation with the emergence rates in 7th, 14th, 28th and 70th day. As for the seeds stored for more than 20 years, the storing years had close correlation with the emergence rates in 7th and 14th day. Above all, it is better to carry out propagation and regeneration when the tobacco budding rates are from 30% to 50%, it is necessary for breeders to take into account of its seed reproduction, so as to prevent the loss of germplasm resources.