Study on the Total RNA Extraction from Young Leaves of Bergenia purpurascens

In order to screen out the best method of extracting total RNA from young leaves of Bergenia purpurascens and provide technique support for RNA-sequencing, six reagent kits such as Trizol kit, Trizol A+ Kit,Transzol Plant Kit, Transzol Up Kit, EasyPure Plant RNA Kit and inunPREP Plant RNA Kit were used and the quality and purity of the total RNAs extracted with them were tested by gel electrophoresis and BIO-RAD SmartSpec Plus. The results showed that RNAs extracted with Trizol Kit、Trizol A+ Kit and Transzol Up Kit lacked the bands of 28S rRNA and 18S rRNA. The RNA extracted with inunPREP Plant RNA Kit showed two weak bands of 28S rRNA and 18S rRNA. The RNAs extracted by Transzol Plant and EasyPure Plant RNA Kit exhibited two bright bands of 28S rRNA and 18S rRNA, but the purity and yield rate of the latter were better. Extraction with EasyPure Plant RNA Kit was the best method to extract the total RNA from young leaves of B. purpurascens.