Study on Improving Tobacco Quality by Using Bacillus siamensis

In order to improve the quality of tobacco, a strain of Bacillus siamensis V16 was isolated and screened from the surface of flue-cured tobacco leaves, the microbial agent prepared from it was sprayed on cut tobacco, treatment method was also optimized. Results showed that after 48 h treatment at 37℃, total sugar, reducing sugar in the treated tobacco increased, while those of nicotine and total nitrogen decreased. Total amount of aromatic substance also increased. Sensory evaluation showed that microbial preparation treatment could reduce offensive irritancy while improve aroma quantity and finish. The T1 got the best result of chemical composition and aroma substances, while T2 showed the best sensory quality. B.siamensis V16 could improve internal quality of tobacco and smoking taste obviously.