Asepsis Sowing and Technology of Rapid Culture and Propagation for Bletilla formosana

To study the technology of rapid propagation for Bletilla formosana, an asepsis sowing technical system was established in greenhouse under natural conditions by using fruits of B. formosana as explants. The research revealed that fruits of B.formosana whose embryo age is 15 weeks were the best explants. The success rate of disinfectant and sterilization was high. In medium of 1/2 MS+NAA 0.3 mg/L+6-BA 0.1 mg/L and 3% sugar, the germination rate was 90%. In the best differentiation medium of MS+NAA 0.5 mg/L+BA 1.0 mg/L+2-iP 0.8 mg/L and 3% sugar, the differentiation rate was 94.5%. In the best rooting medium of 1/2 MS+NAA 1.0 mg/L, 10% banana and 3% sugar, the rooting rate was 100%, plants are healthy which are fostered after 60 days. In Kunming area,B.formosana cultured in greenhouse was transplanted to field and the survival rate was 95%. It has broad prospects for development.