Research on Column Chromatography Separation of Sulforaphane with Nine Kinds of Macroporous Resins

Sulforaphane is a kind of natural anticancer active substances and has good application prospect. We studied the chromatography separation with nine kinds of macroporous resins for sulforaphane of broccoli seeds extract. Firstly, the static adsorption and desorption rate of sulforaphane of broccoli seeds extract with nine kinds of macroporous resins were investigated,and then the recovery rate, purity and cost with the selected three kinds of resins were examined.Finally,the best dynamic parameter of selected resin was selected. The results showed that AB-8 and SP825,SP850 resins had higher static adsorption and desorption rate of nine kinds of mactoporous resins.Through the contrast,AB-8 resin for column chromatography of sulforaphane were selected,the parameters were as follows:the maximum adsorption quantity is 20.07 mg/g;2BV 30% ethanol to wash,4BV 50% ethanol to elute;best diameter ratio of resin column is 1:8.Therefore,separation of sulforaphane can use AB-8 resin,which not only has the good effect but also low cost,it is suitable for industrial production.